LOCUS       BBa_K1100016             795 bp    DNA     linear       01-Jun-2024
DEFINITION  R0040 with insulator and sfGFP
ACCESSION   BBa_K1100016
VERSION     BBa_K1100016.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     stem_loop       69..84
                     /label=insulator: Csy4 cleavage loci
     RBS             84..96
                     /label=B0034
     CDS             103..801
                     /label=sfGFP
     misc_feature    63..795
                     /label=BBa_K1100006
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agctgccgta taggcagcaa agaggagaaa tactagatga gcaaaggcga agaactgttt
      121 accggcgtgg tgccgattct ggttgaactg gatggcgatg ttaatggcca taagtttagc
      181 gtgcgcggcg aaggcgaagg cgatgcgacc aacggcaaac tgaccctgaa gtttatttgc
      241 accaccggca aactgccggt gccgtggccg accctggtga ccaccctgac ctatggcgtg
      301 cagtgcttta gccgctatcc ggatcacatg aaacgccatg atttctttaa gtccgcgatg
      361 ccggaaggct atgttcagga acgcaccatt agctttaaag atgatggcac ctataagacc
      421 cgcgcggaag tgaaatttga aggcgatacc ctggtgaacc gcattgaact gaaaggcatt
      481 gacttcaaag aagatggcaa cattctgggc cataaactgg agtacaattt caacagccat
      541 aacgtgtata ttaccgcgga caaacagaag aacggcatca aagcgaattt caaaatccgc
      601 cataatgtgg aagatggcag cgtgcagctg gcggatcact atcagcagaa taccccgatc
      661 ggcgatggcc cggtgctgct gccggacaat cactacctga gcacccagtc cgtgctgagc
      721 aaagatccga atgagaaacg cgatcacatg gttctgctgg agtttgtgac cgcggcgggt
      781 atcactcatg gctaa
//