BBa_K1100006 1 BBa_K1100006 optimized sfGFP with Csy4 insulator loci 2013-09-12T11:00:00Z 2015-05-08T01:09:08Z The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements. false false _1410_ 0 14489 9 Not in stock false Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated false Hao-tian Guo annotation2339478 1 insulator: Csy4 cleavage loci range2339478 1 7 22 annotation2339688 1 sfGFP range2339688 1 41 739 annotation2339687 1 B0034 range2339687 1 22 34 BBa_K1100016 1 BBa_K1100016 R0040 with insulator and sfGFP 2013-09-12T11:00:00Z 2015-05-08T01:09:08Z coming soon coming soon false false _1410_ 0 14489 9 It's complicated false coming soon false Chenxi He, Jiadong Ni component2341101 1 BBa_K1100006 component2341093 1 BBa_R0040 annotation2341093 1 BBa_R0040 range2341093 1 1 54 annotation2341101 1 BBa_K1100006 range2341101 1 63 795 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986785 1 -35 range1986785 1 20 25 BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K1100016_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa BBa_K1100006_sequence 1 ctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z