LOCUS       BBa_K1100017             892 bp    DNA     linear       23-Dec-2024
DEFINITION  R0063 with insulator and sfGFP
ACCESSION   BBa_K1100017
VERSION     BBa_K1100017.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     stem_loop       166..181
                     /label=insulator: Csy4 cleavage loci
     RBS             181..193
                     /label=B0034
     CDS             200..898
                     /label=sfGFP
     misc_feature    160..892
                     /label=BBa_K1100006
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagagc tgccgtatag gcagcaaaga
      181 ggagaaatac tagatgagca aaggcgaaga actgtttacc ggcgtggtgc cgattctggt
      241 tgaactggat ggcgatgtta atggccataa gtttagcgtg cgcggcgaag gcgaaggcga
      301 tgcgaccaac ggcaaactga ccctgaagtt tatttgcacc accggcaaac tgccggtgcc
      361 gtggccgacc ctggtgacca ccctgaccta tggcgtgcag tgctttagcc gctatccgga
      421 tcacatgaaa cgccatgatt tctttaagtc cgcgatgccg gaaggctatg ttcaggaacg
      481 caccattagc tttaaagatg atggcaccta taagacccgc gcggaagtga aatttgaagg
      541 cgataccctg gtgaaccgca ttgaactgaa aggcattgac ttcaaagaag atggcaacat
      601 tctgggccat aaactggagt acaatttcaa cagccataac gtgtatatta ccgcggacaa
      661 acagaagaac ggcatcaaag cgaatttcaa aatccgccat aatgtggaag atggcagcgt
      721 gcagctggcg gatcactatc agcagaatac cccgatcggc gatggcccgg tgctgctgcc
      781 ggacaatcac tacctgagca cccagtccgt gctgagcaaa gatccgaatg agaaacgcga
      841 tcacatggtt ctgctggagt ttgtgaccgc ggcgggtatc actcatggct aa
//