LOCUS       BBa_K1100018             790 bp    DNA     linear       13-Mar-2025
DEFINITION  R1051 with insulator and sfGFP
ACCESSION   BBa_K1100018
VERSION     BBa_K1100018.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     stem_loop       64..79
                     /label=insulator: Csy4 cleavage loci
     RBS             79..91
                     /label=B0034
     CDS             98..796
                     /label=sfGFP
     misc_feature    58..790
                     /label=BBa_K1100006
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagctg
       61 ccgtataggc agcaaagagg agaaatacta gatgagcaaa ggcgaagaac tgtttaccgg
      121 cgtggtgccg attctggttg aactggatgg cgatgttaat ggccataagt ttagcgtgcg
      181 cggcgaaggc gaaggcgatg cgaccaacgg caaactgacc ctgaagttta tttgcaccac
      241 cggcaaactg ccggtgccgt ggccgaccct ggtgaccacc ctgacctatg gcgtgcagtg
      301 ctttagccgc tatccggatc acatgaaacg ccatgatttc tttaagtccg cgatgccgga
      361 aggctatgtt caggaacgca ccattagctt taaagatgat ggcacctata agacccgcgc
      421 ggaagtgaaa tttgaaggcg ataccctggt gaaccgcatt gaactgaaag gcattgactt
      481 caaagaagat ggcaacattc tgggccataa actggagtac aatttcaaca gccataacgt
      541 gtatattacc gcggacaaac agaagaacgg catcaaagcg aatttcaaaa tccgccataa
      601 tgtggaagat ggcagcgtgc agctggcgga tcactatcag cagaataccc cgatcggcga
      661 tggcccggtg ctgctgccgg acaatcacta cctgagcacc cagtccgtgc tgagcaaaga
      721 tccgaatgag aaacgcgatc acatggttct gctggagttt gtgaccgcgg cgggtatcac
      781 tcatggctaa
//