LOCUS       BBa_K1100019             797 bp    DNA     linear       11-May-2025
DEFINITION  R1062 with insulator and sfGFP
ACCESSION   BBa_K1100019
VERSION     BBa_K1100019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        42..48
                     /label=-10
     misc_feature    54..54
                     /label=start
     promoter        20..25
                     /label=-35
     misc_feature    1..56
                     /label=BBa_R1062
     promoter        1..56
                     /label=BBa_R1062
     stem_loop       71..86
                     /label=insulator: Csy4 cleavage loci
     RBS             86..98
                     /label=B0034
     CDS             105..803
                     /label=sfGFP
     misc_feature    65..797
                     /label=BBa_K1100006
ORIGIN
        1 acctgtagga tcgtacaggt tgacacaaga aaatggtttg ttgatactcg aataaatact
       61 agagctgccg tataggcagc aaagaggaga aatactagat gagcaaaggc gaagaactgt
      121 ttaccggcgt ggtgccgatt ctggttgaac tggatggcga tgttaatggc cataagttta
      181 gcgtgcgcgg cgaaggcgaa ggcgatgcga ccaacggcaa actgaccctg aagtttattt
      241 gcaccaccgg caaactgccg gtgccgtggc cgaccctggt gaccaccctg acctatggcg
      301 tgcagtgctt tagccgctat ccggatcaca tgaaacgcca tgatttcttt aagtccgcga
      361 tgccggaagg ctatgttcag gaacgcacca ttagctttaa agatgatggc acctataaga
      421 cccgcgcgga agtgaaattt gaaggcgata ccctggtgaa ccgcattgaa ctgaaaggca
      481 ttgacttcaa agaagatggc aacattctgg gccataaact ggagtacaat ttcaacagcc
      541 ataacgtgta tattaccgcg gacaaacaga agaacggcat caaagcgaat ttcaaaatcc
      601 gccataatgt ggaagatggc agcgtgcagc tggcggatca ctatcagcag aataccccga
      661 tcggcgatgg cccggtgctg ctgccggaca atcactacct gagcacccag tccgtgctga
      721 gcaaagatcc gaatgagaaa cgcgatcaca tggttctgct ggagtttgtg accgcggcgg
      781 gtatcactca tggctaa
//