BBa_K1100000
1
ALeader
ALeader
2013-09-12T11:00:00Z
2015-05-08T01:09:08Z
coming soon
coming soon
false
false
_1410_
0
14489
9
It's complicated
true
coming soon
false
Hao-tian Guo
annotation2341356
1
ATG
range2341356
1
73
75
annotation2354811
1
ORF11
range2354811
1
16
51
annotation2354810
1
ATG
range2354810
1
13
15
annotation2341355
1
antiSD
range2341355
1
66
69
annotation2341353
1
SD1
range2341353
1
1
4
annotation2341354
1
SD2
range2341354
1
59
62
BBa_K1100022
1
BBa_K1100022
R0063-ALeader
2013-09-26T11:00:00Z
2015-05-08T01:09:08Z
Coming soon
Coming soon
false
false
_1410_
0
18288
9
It's complicated
false
Coming soon
false
Chenxi He
component2364923
1
BBa_K1100000
component2364912
1
BBa_R0063
annotation2364923
1
BBa_K1100000
range2364923
1
160
234
annotation2364912
1
BBa_R0063
range2364912
1
1
151
BBa_R0063
1
lux pL
Promoter (luxR & HSL regulated -- lux pL)<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri.</em>
Released HQ 2013
The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl</p>
false
true
_1_
0
24
7
In stock
false
<P> <P> This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins. <p><img src="<bb_file>Image01.gif</bb_file>" width="614" height="362"><P>Unspecified.
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2051
1
LuxR/HSL
range2051
1
1
20
annotation2053
1
-35
range2053
1
89
94
annotation7071
1
BBa_R0063
range7071
1
1
151
annotation2055
1
Putative LuxR/HSL
range2055
1
130
149
annotation2054
1
start
range2054
1
128
128
annotation2052
1
-10
range2052
1
115
122
BBa_K1100022_sequence
1
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggcagcacggagacacttcagcatg
BBa_R0063_sequence
1
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaa
BBa_K1100000_sequence
1
ggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggcagcacggagacacttcagcatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z