BBa_K1100101 1 BBa_K1100101 J23101 with insulator and sfGFP 2013-09-12T11:00:00Z 2015-05-08T01:09:09Z coming soon coming soon false false _1410_ 0 14489 9 It's complicated false coming soon false Hao-tian Guo component2341151 1 BBa_J23101 component2341155 1 BBa_K1100006 annotation2341151 1 BBa_J23101 range2341151 1 1 35 annotation2341155 1 BBa_K1100006 range2341155 1 44 776 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K1100006 1 BBa_K1100006 optimized sfGFP with Csy4 insulator loci 2013-09-12T11:00:00Z 2015-05-08T01:09:08Z The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab. Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements. false false _1410_ 0 14489 9 Not in stock false Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated false Hao-tian Guo annotation2339478 1 insulator: Csy4 cleavage loci range2339478 1 7 22 annotation2339688 1 sfGFP range2339688 1 41 739 annotation2339687 1 B0034 range2339687 1 22 34 BBa_K1100101_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_K1100006_sequence 1 ctgccgtataggcagcaaagaggagaaatactagatgagcaaaggcgaagaactgtttaccggcgtggtgccgattctggttgaactggatggcgatgttaatggccataagtttagcgtgcgcggcgaaggcgaaggcgatgcgaccaacggcaaactgaccctgaagtttatttgcaccaccggcaaactgccggtgccgtggccgaccctggtgaccaccctgacctatggcgtgcagtgctttagccgctatccggatcacatgaaacgccatgatttctttaagtccgcgatgccggaaggctatgttcaggaacgcaccattagctttaaagatgatggcacctataagacccgcgcggaagtgaaatttgaaggcgataccctggtgaaccgcattgaactgaaaggcattgacttcaaagaagatggcaacattctgggccataaactggagtacaatttcaacagccataacgtgtatattaccgcggacaaacagaagaacggcatcaaagcgaatttcaaaatccgccataatgtggaagatggcagcgtgcagctggcggatcactatcagcagaataccccgatcggcgatggcccggtgctgctgccggacaatcactacctgagcacccagtccgtgctgagcaaagatccgaatgagaaacgcgatcacatggttctgctggagtttgtgaccgcggcgggtatcactcatggctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z