LOCUS       BBa_K1104102            1543 bp    DNA     linear       22-Dec-2024
DEFINITION  pLac+RFP-FimH
ACCESSION   BBa_K1104102
VERSION     BBa_K1104102.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     stem_loop       1426..1469
                     /label=stem_loop
     misc_feature    1415..1494
                     /label=BBa_B0010
     terminator      1415..1494
                     /label=BBa_B0010
     stem_loop       1510..1529
                     /label=T7 TE
     misc_feature    1536..1536
                     /label=stop
     polyA_site      1530..1543
                     /label=polya
     misc_feature    1503..1543
                     /label=BBa_B0012
     terminator      1503..1543
                     /label=BBa_B0012
     terminator      1415..1543
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg cttcctccga
      241 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      301 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      361 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      421 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      481 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      541 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      601 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      661 ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct
      721 gaaactgaaa gacggtggtc actacgacgc tgaagttaaa accacctaca tggctaaaaa
      781 accggttcag ctgccgggtg cttacaaaac cgacatcaaa ctggacatca cctcccacaa
      841 cgaagactac accatcgttg aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc
      901 tttcgcctgt aaaaccgcca atggtaccgc tatccctatt ggcggtggca gcgccaatgt
      961 ttatgtaaac cttgcgcccg tcgtgaatgt ggggcaaaac ctggtcgtgg atctttcgac
     1021 gcaaatcttt tgccataacg attatccgga aaccattaca gactatgtca cactgcaacg
     1081 aggctcggct tatggcggcg tgttatctaa tttttccggg accgtaaaat atagtggcag
     1141 tagctatcca tttcctacca ccagcgaaac gccgcgcgtt gtttataatt cgagaacgga
     1201 taagccgtgg ccggtggcgc tttatttgac gcctgtgagc agtgcgggcg gggtggcgat
     1261 taaagctggc tcattaattg ccgtgcttat tttgcgacag accaacaact ataacagcga
     1321 tgatttccag tttgtgtgga atatttacgc caataatgat gtggtggtgc ctacttaata
     1381 acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa acgaaaggct
     1441 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1501 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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