BBa_K1104204 1 BBa_K1104204 AhpCp2D1 2013-09-15T11:00:00Z 2015-05-08T01:09:09Z ''E. coli MG1655'' genomic sequence We improve the function of a BioBrick Part: AhpC promoter([http://parts.igem.org/Part:BBa_K362001 K362001])designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG. This part contains 3 promoters: AhpC1, DsbG, AhpC2, 2 TFBSs for OxyR, and coding sequence in the initiate part([http://parts.igem.org/Part:BBa_K362001 K362001])was deleted. false false _1415_ 0 16313 9 In stock false We delete the dsbG coding and following sequence next to dsbG reverse primer, making the functional sequence shorter, so that PCR can be more easy and efficient. false Ting-Yun Chiang annotation2365171 1 ctgcag->ctacag range2365171 1 233 238 annotation2365173 1 DsbGp range2365173 1 285 309 annotation2365176 1 (AhpCp1)-35 range2365176 1 393 398 annotation2365169 1 (AhpCp2)-35 range2365169 1 166 171 annotation2365174 1 OxyR binding site3 range2365174 1 356 372 annotation2365170 1 (AhpCp2)-10 range2365170 1 192 197 annotation2365168 1 OxyR binding site2 range2365168 1 141 157 annotation2365177 1 (AhpCp1)-10 range2365177 1 417 422 annotation2365172 1 g->a range2365172 1 235 235 annotation2365175 1 OxyR binding site4 range2365175 1 378 394 annotation2365167 1 OxyR binding site1 range2365167 1 118 137 BBa_K1104204_sequence 1 caatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctacaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z