BBa_K1104206
1
BBa_K1104206
AhpCpD1
2013-09-15T11:00:00Z
2015-05-08T01:09:09Z
''E. coli MG1655'' genomic sequence
:We improve the function of a BioBrick Part: AhpC promoter([http://parts.igem.org/Part:BBa_K362001 K362001])designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG.
:OxyR binding to the ahpC-proximal site leads to the induction of both dsbG and ahpC transcripts, while OxyR binding to the dsbG-proximal site leads to the induction of a second ahpC transcript. This transcript of ahpC and the transcript of dsbG overlap by over 100 nucleotides.
:The intergenic region between dsbG and ahpC carries two binding sites for OxyR, a dsbG-proximal site, located 54 bp upstream of the dsbG start codon, and an ahpC-proximal site, located 290 bp upstream of the dsbG start codon.
false
false
_1415_
0
16313
9
In stock
false
.
false
Ting-Yun Chiang
annotation2365205
1
OxyR binding site4
range2365205
1
94
110
annotation2365207
1
(AhpCp1)-10
range2365207
1
133
138
annotation2365204
1
OxyR binding site3
range2365204
1
72
88
annotation2365206
1
(AhpCp1)-35
range2365206
1
109
114
annotation2365208
1
DsbGp
range2365208
1
1
25
BBa_K1104206_sequence
1
tggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z