BBa_K1104243
type: ComponentDefinition
persistent identity: BBa K1104243
display id: BBa_K1104243
version: 1
was generated by: iGEM to SBOL conversion
description: AhpCp1000 +E0840
created: 2013-10-03T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: AhpCp1000(http://parts.igem.org/Part:BBa_K1104203). The biobrick device is composed of a AhpCp1000 promotor, a OxyR transcription factor which is activated by ROS, and e GFP coding region E0840(http://parts.igem.org/Part:BBa_E0840)
discontinued: false
dominant: false
experience: experience/Works
group u list: _1415_
m user id: 0
owner id: 16313
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Ting-Yun Chiang
type: DnaRegion
role: partType/Measurement
role: SO:0000804
component: BBa_K1104203
component: BBa_E0840
sequence annotation: BBa_K1104203
sequence annotation: BBa_E0840
sequence: https://synbiohub.org/public/igem/BBa_K1104243_sequence/1

BBa_K1104203
type: Component
persistent identity: component2368851
display id: component2368851
version: 1
top level: BBa_K1104243
definition: BBa_K1104203
access: public

BBa_E0840
type: Component
persistent identity: component2368862
display id: component2368862
version: 1
top level: BBa_K1104243
definition: GFP genera
access: public

BBa_K1104203
type: SequenceAnnotation
persistent identity: annotation2368851
display id: annotation2368851
version: 1
top level: BBa_K1104243
location: https://synbiohub.org/public/igem/BBa_K1104243/annotation2368851/range2368851/1
component: BBa_K1104203

https://synbiohub.org/public/igem/BBa_K1104243/annotation2368851/range2368851/1
type: Range
persistent identity: range2368851
display id: range2368851
version: 1
top level: BBa_K1104243
direction: direction/reverse
start: 1
end: 1000
orientation: inline

BBa_E0840
type: SequenceAnnotation
persistent identity: annotation2368862
display id: annotation2368862
version: 1
top level: BBa_K1104243
location: https://synbiohub.org/public/igem/BBa_K1104243/annotation2368862/range2368862/1
component: BBa_E0840

https://synbiohub.org/public/igem/BBa_K1104243/annotation2368862/range2368862/1
type: Range
persistent identity: range2368862
display id: range2368862
version: 1
top level: BBa_K1104243
direction: direction/reverse
start: 1009
end: 1886
orientation: inline

GFP genera
type: ComponentDefinition
persistent identity: BBa E0840
display id: BBa_E0840
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0030.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0030
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0840_sequence/1

BBa_B0030
type: Component
persistent identity: component1249239
display id: component1249239
version: 1
top level: GFP genera
definition: BBa_B0030
access: public

BBa_B0010
type: Component
persistent identity: component1249247
display id: component1249247
version: 1
top level: GFP genera
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249242
display id: component1249242
version: 1
top level: GFP genera
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249257
display id: component1249257
version: 1
top level: GFP genera
definition: BBa_B0012
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1249239
display id: annotation1249239
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_E0840/annotation1249239/range1249239/1
type: Range
persistent identity: range1249239
display id: range1249239
version: 1
top level: GFP genera
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249242
display id: annotation1249242
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0840/annotation1249242/range1249242/1
type: Range
persistent identity: range1249242
display id: range1249242
version: 1
top level: GFP genera
direction: direction/forward
start: 22
end: 741
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249247
display id: annotation1249247
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0840/annotation1249247/range1249247/1
type: Range
persistent identity: range1249247
display id: range1249247
version: 1
top level: GFP genera
direction: direction/forward
start: 750
end: 829
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249257
display id: annotation1249257
version: 1
top level: GFP genera
location: https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0840/annotation1249257/range1249257/1
type: Range
persistent identity: range1249257
display id: range1249257
version: 1
top level: GFP genera
direction: direction/forward
start: 838
end: 878
orientation: inline

BBa_K1104203
type: ComponentDefinition
persistent identity: BBa K1104203
display id: BBa_K1104203
version: 1
was generated by: iGEM to SBOL conversion
description: AhpCp1000
created: 2013-09-15T11:00:00Z
modified: 2015-05-08T01:09:09Z
mutable provenance: E. coli MG1655 genomic sequence
owned by: james
owned by: myers
mutable description: We improve the function of a BioBrick Part: AhpC promoter([http://parts.igem.org/Part:BBa_K362001 K362001])designed by [http://2010.igem.org/Team:KIT-Kyoto/Parts 2010 KIT-Tokyo team] into four versions.On PartRegistry, the complex part(according to [http://ecocyc.org/ECOLI/new-image?object=EG11384 Ecocyc]) composition contains hybrid promoters, shared TFBS (Transcription Factor Binding Site), and reverse promoter DsbG(Figure. 6).In this part AhpC(mutated), we succesfully mutated the Pst1 site (ctgcag->ctacag) of K362001.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1415_
m user id: 0
owner id: 16313
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: AhpC promoter([http://parts.igem.org/Part:BBa_K362001 K362001])has a Pst1 site in the sequence. We designed this part AhpC(mutated), and succesfully mutated the Pst1 site (ctgcag->ctacag) of K362001.
star: false
creator: Ting-Yun Chiang
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: (part of)DsbG coding
sequence annotation: start
sequence annotation: OxyR binding site1
sequence annotation: OxyR binding site2
sequence annotation: -35(AhpCp2)
sequence annotation: -10(AhpCp2)
sequence annotation: AhpCp2
sequence annotation: ctgcag->ctacag
sequence annotation: g->a
sequence annotation: DsbGp
sequence annotation: OxyR binding site3
sequence annotation: OxyR binding site4
sequence annotation: -35(AhpCp1)
sequence annotation: -10(AhpCp1)
sequence annotation: AhpCp1
sequence: https://synbiohub.org/public/igem/BBa_K1104203_sequence/1

(part of)DsbG coding
type: SequenceAnnotation
persistent identity: annotation2366908
display id: annotation2366908
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366908/range2366908/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366908/range2366908/1
type: Range
persistent identity: range2366908
display id: range2366908
version: 1
top level: BBa_K1104203
direction: direction/reverse
start: 1
end: 629
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2366909
display id: annotation2366909
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366909/range2366909/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366909/range2366909/1
type: Range
persistent identity: range2366909
display id: range2366909
version: 1
top level: BBa_K1104203
direction: direction/reverse
start: 627
end: 629
orientation: inline

OxyR binding site1
type: SequenceAnnotation
persistent identity: annotation2366910
display id: annotation2366910
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366910/range2366910/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366910/range2366910/1
type: Range
persistent identity: range2366910
display id: range2366910
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 665
end: 684
orientation: inline

OxyR binding site2
type: SequenceAnnotation
persistent identity: annotation2366911
display id: annotation2366911
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366911/range2366911/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366911/range2366911/1
type: Range
persistent identity: range2366911
display id: range2366911
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 688
end: 704
orientation: inline

-35(AhpCp2)
type: SequenceAnnotation
persistent identity: annotation2366912
display id: annotation2366912
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366912/range2366912/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366912/range2366912/1
type: Range
persistent identity: range2366912
display id: range2366912
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 713
end: 718
orientation: inline

-10(AhpCp2)
type: SequenceAnnotation
persistent identity: annotation2366913
display id: annotation2366913
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366913/range2366913/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366913/range2366913/1
type: Range
persistent identity: range2366913
display id: range2366913
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 739
end: 744
orientation: inline

AhpCp2
type: SequenceAnnotation
persistent identity: annotation2366914
display id: annotation2366914
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366914/range2366914/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366914/range2366914/1
type: Range
persistent identity: range2366914
display id: range2366914
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 749
end: 773
orientation: inline

ctgcag->ctacag
type: SequenceAnnotation
persistent identity: annotation2366915
display id: annotation2366915
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366915/range2366915/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366915/range2366915/1
type: Range
persistent identity: range2366915
display id: range2366915
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 780
end: 785
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation2366916
display id: annotation2366916
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366916/range2366916/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366916/range2366916/1
type: Range
persistent identity: range2366916
display id: range2366916
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 782
end: 782
orientation: inline

DsbGp
type: SequenceAnnotation
persistent identity: annotation2366917
display id: annotation2366917
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366917/range2366917/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366917/range2366917/1
type: Range
persistent identity: range2366917
display id: range2366917
version: 1
top level: BBa_K1104203
direction: direction/reverse
start: 832
end: 856
orientation: inline

OxyR binding site3
type: SequenceAnnotation
persistent identity: annotation2366918
display id: annotation2366918
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366918/range2366918/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366918/range2366918/1
type: Range
persistent identity: range2366918
display id: range2366918
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 903
end: 919
orientation: inline

OxyR binding site4
type: SequenceAnnotation
persistent identity: annotation2366919
display id: annotation2366919
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366919/range2366919/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366919/range2366919/1
type: Range
persistent identity: range2366919
display id: range2366919
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 925
end: 941
orientation: inline

-35(AhpCp1)
type: SequenceAnnotation
persistent identity: annotation2366920
display id: annotation2366920
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366920/range2366920/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366920/range2366920/1
type: Range
persistent identity: range2366920
display id: range2366920
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 940
end: 945
orientation: inline

-10(AhpCp1)
type: SequenceAnnotation
persistent identity: annotation2366921
display id: annotation2366921
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366921/range2366921/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366921/range2366921/1
type: Range
persistent identity: range2366921
display id: range2366921
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 964
end: 969
orientation: inline

AhpCp1
type: SequenceAnnotation
persistent identity: annotation2366922
display id: annotation2366922
version: 1
top level: BBa_K1104203
location: https://synbiohub.org/public/igem/BBa_K1104203/annotation2366922/range2366922/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1104203/annotation2366922/range2366922/1
type: Range
persistent identity: range2366922
display id: range2366922
version: 1
top level: BBa_K1104203
direction: direction/forward
start: 977
end: 1000
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K1104243_sequence/1
type: Sequence
persistent identity: BBa K1104243 sequence
display id: BBa_K1104243_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctacaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatagtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0840_sequence/1
type: Sequence
persistent identity: BBa E0840 sequence
display id: BBa_E0840_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1104203_sequence/1
type: Sequence
persistent identity: BBa K1104203 sequence
display id: BBa_K1104203_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccatcagtttctcattgtcacttaacactttcatttgctctgtacttacgtttgcaggcacgtttagcttaagcttgccaccagaggcttcatattgttgccaggtttttgcgggatctttggaggcaagaattgccgctgctgtcgccgggctttctggcttgataaccccaaccaacaatgttcttaattgcactttgccagaatctacccacgggcgcgcctgctgccagaactgtttacaatatgggcagaacggatcggcgaagacgtagacaatgaccggcgcatcttttttaccgtcgaggagccagtgggattgttccatccgttgccacatttcgcgtccggctggtgcgtaaatttctttttcgataagtgtgttactcaggttttcacctttctcgttgtacatgtaaccagagatagcgtgcttaccatctggagtcaggtagatggtgacgcccatatcctgatactttccgagataacctttcattcctccgggggcatcgaatgttttgatgattgtaatgccctgtttttcaatcgcttttactggagcaggaagttcctctgcgaaggcgattgcaggaagcagagccagtaaaagtatcttttttaacattaatttgtccttttcagtcagtgcaaaagtcgagtaaaaggcataacctatcactgtcataggtaagagcttagatcaggtgattgccctttgtttatgagggtgttgtaatccatgtcgttgttgcatttgtaagggcaacacctcagcctacaggcaggcactgaagataccaaagggtagttcagattacacggtcacctggaaagggggccattttactttttatcgccgctggcggtgcaaagttcacaaagttgtcttacgaaggttgtaaggtaaaacttatcgatttgataatggaaacgcattagccgaatcggcaaaaattggttaccttacatctcatcgaaaacacggaggaagtatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z