BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
BBa_K1104248
1
BBa_K1104248
pSufA-E0840
2013-09-16T11:00:00Z
2015-05-08T01:09:10Z
pSufA comes from the genomix DNA of the MG1655 strain of E.coli, ligaiong with BBa_E0840(http://parts.igem.org/Part:BBa_E0840)
The biobrick device has a sufA promotor which has sensitivity to oxidative stress,will cause the expression of the reporter gene, Green fluorescence protein (GFP E0840).
false
false
_1415_
0
16312
9
In stock
false
none
false
Wan-Hsin Liu
component2349142
1
BBa_K362005
component2349153
1
BBa_E0840
annotation2349153
1
BBa_E0840
range2349153
1
1009
1886
annotation2349142
1
BBa_K362005
range2349142
1
1
1000
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
BBa_K362005
1
BBa_K362005
sufA , this promoter is controlled by OxyR.
2010-09-24T11:00:00Z
2015-05-08T01:12:12Z
sufA , this promoter has sensitivity to oxidative stress.
sufA , this promoter has sensitivity to oxidative stress.
false
false
_480_
0
6267
9
Not in stock
true
sufA , this promoter has sensitivity to oxidative stress.
false
Fumihiko Takeuchi,Aya Iwaki,Yuki Fukuyama,Satori Furumiti, Kazuma Usui, Akiko Kawashima, Toyokazu Nakamura,Reo Yoshimura
BBa_E0840
1
GFP genera
GFP generator
2004-10-17T11:00:00Z
2015-08-31T04:07:26Z
Released HQ 2013
B0030.E0040.B0015
false
true
_11_1_
0
61
7
In stock
true
true
Jennifer Braff
component1249242
1
BBa_E0040
component1249257
1
BBa_B0012
component1249247
1
BBa_B0010
component1249239
1
BBa_B0030
annotation1249239
1
BBa_B0030
range1249239
1
1
15
annotation1249242
1
BBa_E0040
range1249242
1
22
741
annotation1249257
1
BBa_B0012
range1249257
1
838
878
annotation1249247
1
BBa_B0010
range1249247
1
750
829
BBa_E0040
1
GFP
green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
2004-09-29T11:00:00Z
2016-01-26T02:09:38Z
Released HQ 2013
GFP (mut3b) [note that this part does not have a barcode]
false
true
_11_1_
4206
61
7
In stock
false
true
jcbraff
annotation1934520
1
GFP protein
range1934520
1
1
720
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_E0040_sequence
1
atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
BBa_E0840_sequence
1
attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1104248_sequence
1
ctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcacttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctgaaagcgatgaagtgaggtaaatcgtactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K362005_sequence
1
ctggaaatcaatgctaaccacgtccgctcggcacgagaagggcgggtgcgcggcgtatgcaaaccgttgcatctcggttcgcgtcaccaggtctggcagattgaaatcttcgatgagaaagggcgtttgtgctgttcgtcacgattgacgaccgccattttgtgacgttgtcaccagaaaagtgtgacgcatattgcttttggtcattatctgcacagtaaacggtggtatactgtgcaggtcttgtctaaaagcgaggatgctatgtctactcaacttgatcccacccagctggccattgaatttttacgccgtgaccagagcaatctttcacctgcccaatatctcaaacgtctgaaacaactagaacttgaatttgctgacctgcttactctctcttctgctgaattaaaagaagaaatctactttgcctggcgtctgggcgtgcattgatcctgacttaatgccgcaagttcgccaaatcgacctggattaacctccgtccaggttcgatgaccttcttaatactgcttatctaccgcctgatattgttcaaaaataatactaatcgcagtttgtgttaaaacggcgggttagctttatgagattattcttatcgccccttcaagagctaagccactgagagtgccggagataagcgccggatggggtagaaacccttaagcctgtgtcgcacagacttaagggttttcttatttctgaacgctgcacttttttacctatctcttaaatagtcattttcaatacaactttttctttttcatcacagccttaatgctccttagataataaccattatctaacaatgagatacctaattcttagcgtgcctgttaacccacacatcagggtctatgcttattaaaagcaggcaaaaagcatgttttacggtaaagcccctgcgtttgctgggttgaactgataatcattatcactaacatgctgttatacgctgaaagcgatgaagtgaggtaaatcg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z