LOCUS       BBa_K1104412            1747 bp    DNA     linear       12-Apr-2025
DEFINITION  E0840+I13507
ACCESSION   BBa_K1104412
VERSION     BBa_K1104412.1  
FEATURES             Location/Qualifiers
     RBS             1..15
                     /label=RBS-1\Strong
     RBS             8..12
                     /label=RBS
     misc_feature    1..15
                     /label=BBa_B0030
     RBS             1..15
                     /label=BBa_B0030
     CDS             22..741
                     /label=GFP protein
     CDS             22..741
                     /label=BBa_E0040
     stem_loop       761..804
                     /label=stem_loop
     misc_feature    750..829
                     /label=BBa_B0010
     terminator      750..829
                     /label=BBa_B0010
     stem_loop       845..864
                     /label=T7 TE
     misc_feature    871..871
                     /label=stop
     polyA_site      865..878
                     /label=polya
     misc_feature    838..878
                     /label=BBa_B0012
     terminator      838..878
                     /label=BBa_B0012
     misc_feature    1..878
                     /label=BBa_E0840
     misc_feature    891..894
                     /label=conserved
     RBS             887..898
                     /label=BBa_B0034
     CDS             905..1579
                     /label=mrfp1
     misc_feature    1586..1610
                     /label=Help:Barcodes
     CDS             905..1585
                     /label=BBa_E1010
     stem_loop       1630..1673
                     /label=stem_loop
     misc_feature    1619..1698
                     /label=BBa_B0010
     terminator      1619..1698
                     /label=BBa_B0010
     stem_loop       1714..1733
                     /label=T7 TE
     misc_feature    1740..1740
                     /label=stop
     polyA_site      1734..1747
                     /label=polya
     misc_feature    1707..1747
                     /label=BBa_B0012
     terminator      1707..1747
                     /label=BBa_B0012
     misc_feature    887..1747
                     /label=BBa_I13507
ORIGIN
        1 attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca
       61 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      121 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      181 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      241 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      301 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      361 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      421 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      481 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      541 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      601 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      661 aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg
      721 gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc
      781 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      841 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagaaag aggagaaata
      901 ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga
      961 aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga
     1021 aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga
     1081 catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat
     1141 cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt
     1201 cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat
     1261 ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa
     1321 aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg
     1381 tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac
     1441 cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact
     1501 ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg
     1561 tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc tactagagcc
     1621 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1681 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1741 gtttata
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