LOCUS       BBa_K1104701            1790 bp    DNA     linear       22-Dec-2024
DEFINITION  The control group of positive feedback in suicide group
ACCESSION   BBa_K1104701
VERSION     BBa_K1104701.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     CDS             65..784
                     /label=GFP protein
     CDS             65..784
                     /label=BBa_E0040
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     misc_feature    44..921
                     /label=BBa_E0840
     misc_feature    934..937
                     /label=conserved
     RBS             930..941
                     /label=BBa_B0034
     CDS             948..1622
                     /label=mrfp1
     misc_feature    1629..1653
                     /label=Help:Barcodes
     CDS             948..1628
                     /label=BBa_E1010
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
     misc_feature    930..1790
                     /label=BBa_I13507
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagattaaag aggagaaata
       61 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      121 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      181 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      241 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      301 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      361 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      421 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      481 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      541 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      601 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      661 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      721 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagaga aagaggagaa atactagatg gcttcctccg
      961 aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc
     1021 acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta
     1081 aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt
     1141 tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac
     1201 tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg
     1261 ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc
     1321 gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag
     1381 cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc
     1441 tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa
     1501 aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca
     1561 acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg
     1621 cttaataacg ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//