##gff-version 3
##sequence-region BBa_K1104702 1 7343
BBa_K1104702	.	promoter	3605	3639	.	+	0	ID=BBa_J23102;Name=BBa_J23102
BBa_K1104702	.	ribosome_entry_site	55	66	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1104702	.	conserved_region	59	62	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1104702	.	terminator	7215	7343	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K1104702	.	terminator	7303	7343	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1104702	.	polyA_site	7330	7343	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1104702	.	stem_loop	7310	7329	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1104702	.	stop_codon	7336	7336	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1104702	.	engineered_region	7303	7343	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1104702	.	terminator	7215	7294	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1104702	.	stem_loop	7226	7269	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1104702	.	engineered_region	7215	7294	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1104702	.	engineered_region	2736	3596	.	+	0	ID=BBa_I13507;Name=BBa_I13507
BBa_K1104702	.	CDS	2754	3434	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13507
BBa_K1104702	.	engineered_tag	3435	3459	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1104702	.	CDS	2754	3428	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1104702	.	ribosome_entry_site	2736	2747	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13507
BBa_K1104702	.	conserved_region	2740	2743	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1104702	.	terminator	3468	3547	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13507
BBa_K1104702	.	stem_loop	3479	3522	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1104702	.	engineered_region	3468	3547	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1104702	.	terminator	3556	3596	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13507
BBa_K1104702	.	polyA_site	3583	3596	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1104702	.	stem_loop	3563	3582	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1104702	.	stop_codon	3589	3589	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1104702	.	engineered_region	3556	3596	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1104702	.	CDS	73	2727	.	+	0	ID=BBa_K145001;Name=BBa_K145001
BBa_K1104702	.	CDS	76	2721	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1104702	.	stop_codon	2722	2724	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K1104702	.	start_codon	73	75	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1104702	.	stop_codon	2725	2727	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
BBa_K1104702	.	ribosome_entry_site	4534	4545	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K1104702	.	conserved_region	4538	4541	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1104702	.	engineered_region	3648	4525	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K1104702	.	ribosome_entry_site	3648	3662	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K1104702	.	ribosome_entry_site	3655	3659	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1104702	.	ribosome_entry_site	3648	3662	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1104702	.	engineered_region	3648	3662	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1104702	.	CDS	3669	4388	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K1104702	.	CDS	3669	4388	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K1104702	.	terminator	4485	4525	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K1104702	.	polyA_site	4512	4525	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1104702	.	stem_loop	4492	4511	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1104702	.	stop_codon	4518	4518	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1104702	.	engineered_region	4485	4525	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1104702	.	terminator	4397	4476	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K1104702	.	stem_loop	4408	4451	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1104702	.	engineered_region	4397	4476	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1104702	.	promoter	1	46	.	+	0	ID=BBa_I712074;Name=BBa_I712074
BBa_K1104702	.	CDS	4552	7206	.	+	0	ID=BBa_K145001;Name=BBa_K145001
BBa_K1104702	.	CDS	4555	7200	.	+	0	ID=annotation1968243;Name=protein;Parent=BBa_K145001
BBa_K1104702	.	stop_codon	7201	7203	.	+	0	ID=annotation1968217;Name=stop;Parent=BBa_K145001
BBa_K1104702	.	start_codon	4552	4554	.	+	0	ID=annotation1968216;Name=start;Parent=BBa_K145001
BBa_K1104702	.	stop_codon	7204	7206	.	+	0	ID=annotation1968218;Name=stop;Parent=BBa_K145001
>BBa_K1104702
taatacgactcactatagggaatacaagctacttgttctttttgcatactagagaaagaggagaaatactagatgaaca
cgattaacatcgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacgg
tgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgttt
gagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatga
ttgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaaga
aatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgtt
caggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagc
acttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcga
ggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtagga
gtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaag
actctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctcc
gatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgt
cctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaag
cgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaa
gcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcct
gaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagcc
ttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcgg
tcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaa
ccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctg
agcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggc
tgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataac
tgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtg
gtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattct
acaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaa
gtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtca
tgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattc
cggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtg
acggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataaga
agactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaa
gaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagat
agcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgta
agactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggc
tgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttc
tacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacc
tccgtgacatcttagagtcggacttcgcgttcgcgtaataatactagagaaagaggagaaatactagatggcttcctcc
gaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaag
gtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgc
ttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctg
aaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccagg
actcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttat
gcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaa
atgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagc
tgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacga
acgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtactgtg
ctagctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattctt
gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaac
ttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgt
tcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacag
gaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactc
acacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagat
ggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt
acctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagc
tgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttatatactagagaaagaggagaaatactagatgaacacgattaacatcgctaagaacgact
tctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagtt
ggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgag
gttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttg
aggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgcaagaaatcaagccggaagccgtagcgta
catcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggt
cgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaac
aactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtct
actcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcatt
gagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcac
ctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcc
tcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcac
agtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccg
catggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccc
tgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgt
gctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagcca
ataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgtt
caacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactac
tggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaa
accacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgctt
ccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgac
gggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttccta
gtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggac
cgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactg
gctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaag
agttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttcactca
gccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttgaagca
atgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcgcaagc
gttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgcttgaa
cctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacacaaacag
gagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcacacgaga
agtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttcaaagc
agtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgaccagttg
cacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtcggact
tcgcgttcgcgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata