LOCUS       BBa_K1104903            1898 bp    DNA     linear       07-Dec-2025
DEFINITION  pLux/cI + Kill proteins, LuxI, LuxR generator
ACCESSION   BBa_K1104903
VERSION     BBa_K1104903.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     misc_feature    124..126
                     /label=Methionine
     misc_feature    283..285
                     /label=Term
     CDS             127..282
                     /label=mature peptide
     CDS             124..285
                     /label=BBa_K1104301
     misc_feature    298..301
                     /label=conserved
     RBS             294..305
                     /label=BBa_B0034
     misc_feature    312..313
                     /label=prefix
     misc_feature    485..485
                     /label=T
     misc_feature    803..803
                     /label=A
     CDS             312..1061
                     /label=luxR
     misc_feature    312..1067
                     /label=BBa_C0062
     misc_feature    1068..1092
                     /label=Help:Barcodes
     CDS             312..1067
                     /label=BBa_C0062
     misc_feature    1105..1108
                     /label=conserved
     RBS             1101..1112
                     /label=BBa_B0034
     misc_feature    1698..1729
                     /label=LVA
     CDS             1119..1697
                     /label=luxI
     misc_feature    1119..1736
                     /label=BBa_C0061
     misc_feature    1737..1761
                     /label=Help:Barcodes
     CDS             1119..1736
                     /label=BBa_C0061
     stem_loop       1781..1824
                     /label=stem_loop
     misc_feature    1770..1849
                     /label=BBa_B0010
     terminator      1770..1849
                     /label=BBa_B0010
     stem_loop       1865..1884
                     /label=T7 TE
     misc_feature    1891..1891
                     /label=stop
     polyA_site      1885..1898
                     /label=polya
     misc_feature    1858..1898
                     /label=BBa_B0012
     terminator      1858..1898
                     /label=BBa_B0012
     terminator      1770..1898
                     /label=BBa_B0015
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatggtaa cttgtgacct tctctcattc aaaggacaag ttaatgacag tgcttgcgct
      181 gctaactgtc tcagtttggg taaagctgga ggtcattgcg agaaaggagt ttgtatttgt
      241 cgaaaaacca gtttcaaaga tctctgggac aaacgtttcg gttaatacta gagaaagagg
      301 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta
      361 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac
      421 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt
      481 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa
      541 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat
      601 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag
      661 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta
      721 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga
      781 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata
      841 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag
      901 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc
      961 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag
     1021 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct
     1081 agtgtagatc actactagag aaagaggaga aatactagat gactataatg ataaaaaaat
     1141 cggatttttt ggcaattcca tcggaggagt ataaaggtat tctaagtctt cgttatcaag
     1201 tgtttaagca aagacttgag tgggacttag ttgtagaaaa taaccttgaa tcagatgagt
     1261 atgataactc aaatgcagaa tatatttatg cttgtgatga tactgaaaat gtaagtggat
     1321 gctggcgttt attacctaca acaggtgatt atatgctgaa aagtgttttt cctgaattgc
     1381 ttggtcaaca gagtgctccc aaagatccta atatagtcga attaagtcgt tttgctgtag
     1441 gtaaaaatag ctcaaagata aataactctg ctagtgaaat tacaatgaaa ctatttgaag
     1501 ctatatataa acacgctgtt agtcaaggta ttacagaata tgtaacagta acatcaacag
     1561 caatagagcg atttttaaag cgtattaaag ttccttgtca tcgtattgga gacaaagaaa
     1621 ttcatgtatt aggtgatact aaatcggttg tattgtctat gcctattaat gaacagttta
     1681 aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc tttagtagct taataactct
     1741 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1801 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1861 cactggctca ccttcgggtg ggcctttctg cgtttata
//