BBa_K1105000 1 BBa_K1105000 lacIq-T7-ccdB 2013-09-22T11:00:00Z 2015-07-09T11:36:37Z This part (BBa_K1105000) comes from p10-LacIq-T7ccdB, a plasmid constructed by the lab Inbio-UGent. Originally, it comes from strains which are mini F-plasmid positive, such as E. coli F+. This part contains a ccdB gene under control of a T7-promotor. CcdB is a bacterial toxin that interferes with topoisomerase unit gyrA. The toxin CcdB can be neutralized by its antitoxin CcdA. The T7-promoter allows for the controlled expression of CcdB. The goal of the UGent team 2013 is to improve the existing CIChE technique(Chemically inducible chromosomal evolution),developed by Tyo et al. in 2009. This was done by replacing the antibiotic resistance genes in the genome by CcdA antitoxin genes. This part was used to put pressure on the bacterial cells, in that way inducing chromosomal evolution. true false _1416_ 4206 16040 9 It's complicated false We had problems cloning our part in the pSB1C3 backbone due to the high copy number of pSB1C3. In every attempt mutations occurred. This is probably caused by the high pressure leaky expression of the T7 promoter may put on the cells. In the case of leaky expression, mutants (no ccdB expression)have a huge growth advantage over cells containing the correct. This lead us to submit our part in the pSB6A1 backbone (low copy number). false Eva D annotation2367591 1 ccdB Start codon range2367591 1 1613 1615 annotation2367575 1 pT7 range2367575 1 1526 1542 annotation2367577 1 RBS-1 range2367577 1 1600 1605 annotation2367574 1 LacIq range2367574 1 231 1190 annotation2367576 1 LacO range2367576 1 1547 1567 annotation2367578 1 ccdB range2367578 1 1613 1921 annotation2367572 1 -10box range2367572 1 67 72 annotation2367571 1 -35box range2367571 1 44 49 annotation2367592 1 ccdB Stop codon range2367592 1 1919 1921 annotation2367573 1 LacI range2367573 1 108 230 BBa_K1105000_sequence 1 tgcgcctgatcatgttcgtcatttacgttgacaccatcgaatggtgcaaaacctttcgcggtatggcatgatagcgcccggaagagagtcaattcagggtggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgtcaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgtgatcatcaacgcaattaatgtgagttagcgcgaattgatctggtttgacagcttatcatcgactgcacggtgcaccaatgcttctggcgtcaggcagccatcggaagctgtggtatggctgtgcaggtcgtaaatcactgcataattcgtgtcgctcaaggcgcactcccgttctggataatgttttttgcgccgacatcataacggttctgggtctaggagctcgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaaattaatacgactcactataggggaattgtgagcggataacaattcccctgtagaaataattttgtttaactttaataaggagatataccatggcacagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataacctaggagaagtgaaacgccgtagcgccgatggtagtgtggggtctccccatgcgagagtagggaactgccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcctgagtaggacaaatccgccgggagcggatttgaacgttgcgaagcaacggcccggagggtggcgggcaggacgcccgccataaactgccaggcatcaaattaagcagaaggccatcctgacggatggcctttttc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z