LOCUS       BBa_K1106003            1390 bp    DNA     linear       09-May-2024
DEFINITION  mRFP generator under Ars promoter
ACCESSION   BBa_K1106003
VERSION     BBa_K1106003.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     RBS             527..541
                     /label=RBS-1\Strong
     RBS             534..538
                     /label=RBS
     misc_feature    527..541
                     /label=BBa_B0030
     RBS             527..541
                     /label=BBa_B0030
     CDS             548..1222
                     /label=mrfp1
     misc_feature    1229..1253
                     /label=Help:Barcodes
     CDS             548..1253
                     /label=BBa_E1010
     stem_loop       1273..1316
                     /label=stem_loop
     misc_feature    1262..1341
                     /label=BBa_B0010
     terminator      1262..1341
                     /label=BBa_B0010
     stem_loop       1357..1376
                     /label=T7 TE
     misc_feature    1383..1383
                     /label=stop
     polyA_site      1377..1390
                     /label=polya
     misc_feature    1350..1390
                     /label=BBa_B0012
     terminator      1350..1390
                     /label=BBa_B0012
     terminator      1262..1390
                     /label=BBa_B0015
     misc_feature    527..1390
                     /label=BBa_K516030
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagatta aagaggagaa
      541 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
      601 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      661 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
      721 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
      781 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
      841 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
      901 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
      961 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
     1021 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
     1081 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
     1141 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
     1201 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
     1261 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1321 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1381 tgcgtttata
//