LOCUS       BBa_K1106004            1404 bp    DNA     linear       09-May-2024
DEFINITION  GFP generator under Ars promoter
ACCESSION   BBa_K1106004
VERSION     BBa_K1106004.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     RBS             527..541
                     /label=RBS-1\Strong
     RBS             534..538
                     /label=RBS
     misc_feature    527..541
                     /label=BBa_B0030
     RBS             527..541
                     /label=BBa_B0030
     CDS             548..1267
                     /label=GFP protein
     CDS             548..1267
                     /label=BBa_E0040
     stem_loop       1287..1330
                     /label=stem_loop
     misc_feature    1276..1355
                     /label=BBa_B0010
     terminator      1276..1355
                     /label=BBa_B0010
     stem_loop       1371..1390
                     /label=T7 TE
     misc_feature    1397..1397
                     /label=stop
     polyA_site      1391..1404
                     /label=polya
     misc_feature    1364..1404
                     /label=BBa_B0012
     terminator      1364..1404
                     /label=BBa_B0012
     misc_feature    527..1404
                     /label=BBa_E0840
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagatta aagaggagaa
      541 atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      601 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      661 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      721 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      781 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      841 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      901 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      961 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     1021 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     1081 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1141 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     1201 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     1261 ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1321 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1381 cgggtgggcc tttctgcgtt tata
//