LOCUS       BBa_K1106006            1928 bp    DNA     linear       22-Nov-2024
DEFINITION  Pulse generator part 3
ACCESSION   BBa_K1106006
VERSION     BBa_K1106006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux-box
     promoter        20..25
                     /label=-35
     misc_feature    23..40
                     /label=OR2
     promoter        42..47
                     /label=-10
     misc_feature    46..63
                     /label=OR1
     promoter        1..66
                     /label=BBa_K145150
     RBS             75..89
                     /label=RBS-1\Strong
     RBS             82..86
                     /label=RBS
     misc_feature    75..89
                     /label=BBa_B0030
     RBS             75..89
                     /label=BBa_B0030
     CDS             96..770
                     /label=mrfp1
     misc_feature    777..801
                     /label=Help:Barcodes
     CDS             96..801
                     /label=BBa_E1010
     stem_loop       821..864
                     /label=stem_loop
     misc_feature    810..889
                     /label=BBa_B0010
     terminator      810..889
                     /label=BBa_B0010
     stem_loop       905..924
                     /label=T7 TE
     misc_feature    931..931
                     /label=stop
     polyA_site      925..938
                     /label=polya
     misc_feature    898..938
                     /label=BBa_B0012
     terminator      898..938
                     /label=BBa_B0012
     terminator      810..938
                     /label=BBa_B0015
     misc_feature    75..938
                     /label=BBa_K516030
     promoter        947..981
                     /label=BBa_J23119
     RBS             990..1004
                     /label=RBS-1\Strong
     RBS             997..1001
                     /label=RBS
     misc_feature    990..1004
                     /label=BBa_B0030
     RBS             990..1004
                     /label=BBa_B0030
     misc_feature    1011..1012
                     /label=prefix
     misc_feature    1184..1184
                     /label=T
     misc_feature    1502..1502
                     /label=A
     CDS             1011..1760
                     /label=luxR
     misc_feature    1011..1766
                     /label=BBa_C0062
     misc_feature    1767..1791
                     /label=Help:Barcodes
     CDS             1011..1766
                     /label=BBa_C0062
     stem_loop       1811..1854
                     /label=stem_loop
     misc_feature    1800..1879
                     /label=BBa_B0010
     terminator      1800..1879
                     /label=BBa_B0010
     stem_loop       1895..1914
                     /label=T7 TE
     misc_feature    1921..1921
                     /label=stop
     polyA_site      1915..1928
                     /label=polya
     misc_feature    1888..1928
                     /label=BBa_B0012
     terminator      1888..1928
                     /label=BBa_B0012
     terminator      1800..1928
                     /label=BBa_B0015
     misc_feature    947..1928
                     /label=BBa_K1106005
ORIGIN
        1 acctgtagga tcgtacaggt ttactaaaga ttcctttagt ttataattta agtgttcttt
       61 aatttctact agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
      121 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      181 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      241 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      301 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      361 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      421 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      481 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      541 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
      601 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
      661 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
      721 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
      781 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      841 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      901 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cagctagctc
      961 agtcctaggt ataatgctag ctactagaga ttaaagagga gaaatactag atgaaaaaca
     1021 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     1081 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     1141 cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta
     1201 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     1261 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     1321 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt
     1381 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag
     1441 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     1501 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     1561 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     1621 caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     1681 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     1741 attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc
     1801 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     1861 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     1921 cgtttata
//