##gff-version 3
##sequence-region BBa_K1106006 1 1928
BBa_K1106006	.	engineered_region	947	1928	.	+	0	ID=BBa_K1106005;Name=BBa_K1106005
BBa_K1106006	.	terminator	1800	1928	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1106005
BBa_K1106006	.	terminator	1800	1879	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1106006	.	engineered_region	1800	1879	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1106006	.	stem_loop	1811	1854	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1106006	.	terminator	1888	1928	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1106006	.	engineered_region	1888	1928	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1106006	.	polyA_site	1915	1928	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1106006	.	stem_loop	1895	1914	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1106006	.	stop_codon	1921	1921	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1106006	.	CDS	1011	1766	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_K1106005
BBa_K1106006	.	engineered_tag	1767	1791	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1106006	.	CDS	1011	1760	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1106006	.	point_mutation	1502	1502	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1106006	.	engineered_region	1011	1766	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1106006	.	point_mutation	1184	1184	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1106006	.	engineered_region	1011	1012	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1106006	.	ribosome_entry_site	990	1004	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K1106005
BBa_K1106006	.	ribosome_entry_site	990	1004	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1106006	.	ribosome_entry_site	997	1001	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1106006	.	engineered_region	990	1004	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1106006	.	promoter	947	981	.	+	0	ID=BBa_J23119;Name=BBa_J23119;Parent=BBa_K1106005
BBa_K1106006	.	engineered_region	75	938	.	+	0	ID=BBa_K516030;Name=BBa_K516030
BBa_K1106006	.	terminator	810	938	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K516030
BBa_K1106006	.	terminator	810	889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1106006	.	engineered_region	810	889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1106006	.	stem_loop	821	864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1106006	.	terminator	898	938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1106006	.	engineered_region	898	938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1106006	.	polyA_site	925	938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1106006	.	stem_loop	905	924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1106006	.	stop_codon	931	931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1106006	.	CDS	96	801	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K516030
BBa_K1106006	.	CDS	96	770	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1106006	.	engineered_tag	777	801	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1106006	.	ribosome_entry_site	75	89	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K516030
BBa_K1106006	.	ribosome_entry_site	75	89	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1106006	.	ribosome_entry_site	82	86	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1106006	.	engineered_region	75	89	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1106006	.	promoter	1	66	.	+	0	ID=BBa_K145150;Name=BBa_K145150
BBa_K1106006	.	promoter	42	47	.	+	0	ID=annotation1971585;Name=-10;Parent=BBa_K145150
BBa_K1106006	.	non_covalent_binding_site	23	40	.	+	0	ID=annotation1971584;Name=OR2;Parent=BBa_K145150
BBa_K1106006	.	non_covalent_binding_site	46	63	.	+	0	ID=annotation1971586;Name=OR1;Parent=BBa_K145150
BBa_K1106006	.	promoter	20	25	.	+	0	ID=annotation1971583;Name=-35;Parent=BBa_K145150
BBa_K1106006	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation1971582;Name=Lux-box;Parent=BBa_K145150
>BBa_K1106006
acctgtaggatcgtacaggtttactaaagattcctttagtttataatttaagtgttctttaatttctactagagattaa
agaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttcc
gttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaag
ttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaa
acacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaa
gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggta
ccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgga
agacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaacc
acctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacg
aagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgcta
gtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtt
gacagctagctcagtcctaggtataatgctagctactagagattaaagaggagaaatactagatgaaaaacataaatgc
cgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatg
actaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcc
tagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattc
taactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaa
gaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtt
ttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttc
tctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgt
ttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcc
atttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaat
tgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttata