LOCUS       BBa_K1106007            1415 bp    DNA     linear       29-Nov-2024
DEFINITION  pulse generator part 2
ACCESSION   BBa_K1106007
VERSION     BBa_K1106007.1  
FEATURES             Location/Qualifiers
     promoter        1..92
                     /label=PLL
     promoter        1..92
                     /label=BBa_I746365
     RBS             101..115
                     /label=RBS-1\Strong
     RBS             108..112
                     /label=RBS
     misc_feature    101..115
                     /label=BBa_B0030
     RBS             101..115
                     /label=BBa_B0030
     CDS             122..769
                     /label=cII p22
     misc_feature    770..802
                     /label=LVA
     misc_feature    803..808
                     /label=stop
     misc_feature    122..808
                     /label=BBa_C0053
     misc_feature    809..833
                     /label=Help:Barcodes
     CDS             122..808
                     /label=BBa_C0053
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
     terminator      842..970
                     /label=BBa_B0015
     misc_feature    1..970
                     /label=BBa_K1106001
     misc_feature    979..998
                     /label=LuxR/HSL
     promoter        998..1003
                     /label=-35
     promoter        1020..1025
                     /label=-10
     misc_feature    1031..1031
                     /label=start
     misc_feature    979..1033
                     /label=BBa_R0062
     promoter        979..1033
                     /label=BBa_R0062
     misc_feature    1042..1053
                     /label=B0034
     misc_feature    1060..1060
                     /label=PSP3 pag
     CDS             1060..1278
                     /label=PSP3 pag
     misc_feature    1042..1278
                     /label=BBa_I746351
     stem_loop       1298..1341
                     /label=stem_loop
     misc_feature    1287..1366
                     /label=BBa_B0010
     terminator      1287..1366
                     /label=BBa_B0010
     stem_loop       1382..1401
                     /label=T7 TE
     misc_feature    1408..1408
                     /label=stop
     polyA_site      1402..1415
                     /label=polya
     misc_feature    1375..1415
                     /label=BBa_B0012
     terminator      1375..1415
                     /label=BBa_B0012
     terminator      1287..1415
                     /label=BBa_B0015
     misc_feature    979..1415
                     /label=BBa_K1106002
ORIGIN
        1 gtggagatga acaaaaaaca cacagccatt gtaagacagc ctgaacaaat cccccctgtt
       61 gcgtctgctg aaaatattca caaaataaag cgtactagag attaaagagg agaaatacta
      121 gatgaataca caattgatgg gtgagcgtat tcgcgctcga agaaaaaaac tcaagattag
      181 acaagccgct cttggtaaga tggtgggagt gtctaatgtt gcaatatcgc aatgggagcg
      241 ctcggagact gagccaaatg gggagaacct gttggcactt tcgaaggctc ttcagtgctc
      301 ccctgactat ttgctgaaag gagatttaag ccagacaaac gttgcctatc atagtaggca
      361 tgagccaaga ggatcatacc ctcttatcag ttgggtaagc gcagggcaat ggatggaagc
      421 tgtagaacct tatcacaagc gcgcgataga gaactggcac gacaccactg tagattgttc
      481 agaagattca ttttggcttg atgtccaagg tgactctatg acagcaccgg cagggttaag
      541 cattccagaa ggaatgataa ttctggttga tcccgaagtc gaaccaagaa acggcaagct
      601 ggttgttgca aaattagaag gtgaaaacga ggccacattc aaaaaattag ttatggatgc
      661 aggccgaaag tttttaaaac cattaaaccc acaatatccg atgatagaaa tcaacggaaa
      721 ctgcaaaatc attggcgtag ttgttgacgc aaaactcgca aatcttccag ctgcaaacga
      781 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt
     1021 atagtcgaat aaatactaga gaaagaggag aaatactaga tgatgcactg cccgttatgc
     1081 caaaacgctg cacatgctcg cactagccgg taccttagca ccgaaacgaa agaacgttat
     1141 caccagtgcc aaaacataaa ttgcggatgt acatttatca cttttgagac actatcaaga
     1201 ttcattgtga aaccggggac tgttgatcct gctccgcccc accccatcag aaaccaacaa
     1261 cagcaacttt ggctttgata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1321 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1381 tggctcacct tcgggtgggc ctttctgcgt ttata
//