BBa_K1106007
type: ComponentDefinition
persistent identity: BBa K1106007
display id: BBa_K1106007
version: 1
was generated by: iGEM to SBOL conversion
description: pulse generator part 2
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:10Z
mutable provenance: c
owned by: james
owned by: myers
mutable description: c
discontinued: false
dominant: false
group u list: _1417_
m user id: 0
owner id: 16344
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: c
star: false
creator: In??s Luc??a Patop
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K1106002
component: BBa_K1106001
sequence annotation: BBa_K1106001
sequence annotation: BBa_K1106002
sequence: https://synbiohub.org/public/igem/BBa_K1106007_sequence/1

BBa_K1106002
type: Component
persistent identity: component2376834
display id: component2376834
version: 1
top level: BBa_K1106007
definition: BBa_K1106002
access: public

BBa_K1106001
type: Component
persistent identity: component2376817
display id: component2376817
version: 1
top level: BBa_K1106007
definition: BBa_K1106001
access: public

BBa_K1106001
type: SequenceAnnotation
persistent identity: annotation2376817
display id: annotation2376817
version: 1
top level: BBa_K1106007
location: https://synbiohub.org/public/igem/BBa_K1106007/annotation2376817/range2376817/1
component: BBa_K1106001

https://synbiohub.org/public/igem/BBa_K1106007/annotation2376817/range2376817/1
type: Range
persistent identity: range2376817
display id: range2376817
version: 1
top level: BBa_K1106007
direction: direction/reverse
start: 1
end: 970
orientation: inline

BBa_K1106002
type: SequenceAnnotation
persistent identity: annotation2376834
display id: annotation2376834
version: 1
top level: BBa_K1106007
location: https://synbiohub.org/public/igem/BBa_K1106007/annotation2376834/range2376834/1
component: BBa_K1106002

https://synbiohub.org/public/igem/BBa_K1106007/annotation2376834/range2376834/1
type: Range
persistent identity: range2376834
display id: range2376834
version: 1
top level: BBa_K1106007
direction: direction/reverse
start: 979
end: 1415
orientation: inline

BBa_K1106001
type: ComponentDefinition
persistent identity: BBa K1106001
display id: BBa_K1106001
version: 1
was generated by: iGEM to SBOL conversion
description: P22 c2 generator under PLL promoter
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:10Z
mutable provenance: compl
owned by: james
owned by: myers
mutable description: completar
discontinued: false
dominant: false
experience: experience/None
group u list: _1417_
m user id: 0
owner id: 16344
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: compl
star: false
creator: In??s Luc??a Patop
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I746365
component: BBa_B0015
component: BBa_C0053
component: BBa_B0030
sequence annotation: BBa_I746365
sequence annotation: BBa_B0030
sequence annotation: BBa_C0053
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1106001_sequence/1

BBa_I746365
type: Component
persistent identity: component2373549
display id: component2373549
version: 1
top level: BBa_K1106001
definition: BBa_I746365
access: public

BBa_B0015
type: Component
persistent identity: component2373564
display id: component2373564
version: 1
top level: BBa_K1106001
definition: BBa_B0015
access: public

BBa_C0053
type: Component
persistent identity: component2373554
display id: component2373554
version: 1
top level: BBa_K1106001
definition: c2 P22
access: public

BBa_B0030
type: Component
persistent identity: component2373551
display id: component2373551
version: 1
top level: BBa_K1106001
definition: BBa_B0030
access: public

BBa_I746365
type: SequenceAnnotation
persistent identity: annotation2373549
display id: annotation2373549
version: 1
top level: BBa_K1106001
location: https://synbiohub.org/public/igem/BBa_K1106001/annotation2373549/range2373549/1
component: BBa_I746365

https://synbiohub.org/public/igem/BBa_K1106001/annotation2373549/range2373549/1
type: Range
persistent identity: range2373549
display id: range2373549
version: 1
top level: BBa_K1106001
direction: direction/reverse
start: 1
end: 92
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2373551
display id: annotation2373551
version: 1
top level: BBa_K1106001
location: https://synbiohub.org/public/igem/BBa_K1106001/annotation2373551/range2373551/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1106001/annotation2373551/range2373551/1
type: Range
persistent identity: range2373551
display id: range2373551
version: 1
top level: BBa_K1106001
direction: direction/forward
start: 101
end: 115
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation2373554
display id: annotation2373554
version: 1
top level: BBa_K1106001
location: https://synbiohub.org/public/igem/BBa_K1106001/annotation2373554/range2373554/1
component: BBa_C0053

https://synbiohub.org/public/igem/BBa_K1106001/annotation2373554/range2373554/1
type: Range
persistent identity: range2373554
display id: range2373554
version: 1
top level: BBa_K1106001
direction: direction/forward
start: 122
end: 808
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2373564
display id: annotation2373564
version: 1
top level: BBa_K1106001
location: https://synbiohub.org/public/igem/BBa_K1106001/annotation2373564/range2373564/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1106001/annotation2373564/range2373564/1
type: Range
persistent identity: range2373564
display id: range2373564
version: 1
top level: BBa_K1106001
direction: direction/reverse
start: 842
end: 970
orientation: inline

BBa_K1106002
type: ComponentDefinition
persistent identity: BBa K1106002
display id: BBa_K1106002
version: 1
was generated by: iGEM to SBOL conversion
description: psp3 generator under lux pR promoter
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:10Z
mutable provenance: c
owned by: james
owned by: myers
mutable description: comp
discontinued: false
dominant: false
experience: experience/None
group u list: _1417_
m user id: 0
owner id: 16344
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: c
star: false
creator: In??s Luc??a Patop
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_I746351
component: BBa_R0062
sequence annotation: BBa_R0062
sequence annotation: BBa_I746351
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1106002_sequence/1

BBa_B0015
type: Component
persistent identity: component2337248
display id: component2337248
version: 1
top level: BBa_K1106002
definition: BBa_B0015
access: public

BBa_I746351
type: Component
persistent identity: component2337241
display id: component2337241
version: 1
top level: BBa_K1106002
definition: BBa_I746351
access: public

BBa_R0062
type: Component
persistent identity: component2337233
display id: component2337233
version: 1
top level: BBa_K1106002
definition: lux pR
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2337233
display id: annotation2337233
version: 1
top level: BBa_K1106002
location: https://synbiohub.org/public/igem/BBa_K1106002/annotation2337233/range2337233/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1106002/annotation2337233/range2337233/1
type: Range
persistent identity: range2337233
display id: range2337233
version: 1
top level: BBa_K1106002
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_I746351
type: SequenceAnnotation
persistent identity: annotation2337241
display id: annotation2337241
version: 1
top level: BBa_K1106002
location: https://synbiohub.org/public/igem/BBa_K1106002/annotation2337241/range2337241/1
component: BBa_I746351

https://synbiohub.org/public/igem/BBa_K1106002/annotation2337241/range2337241/1
type: Range
persistent identity: range2337241
display id: range2337241
version: 1
top level: BBa_K1106002
direction: direction/reverse
start: 64
end: 300
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2337248
display id: annotation2337248
version: 1
top level: BBa_K1106002
location: https://synbiohub.org/public/igem/BBa_K1106002/annotation2337248/range2337248/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1106002/annotation2337248/range2337248/1
type: Range
persistent identity: range2337248
display id: range2337248
version: 1
top level: BBa_K1106002
direction: direction/reverse
start: 309
end: 437
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

c2 P22
type: ComponentDefinition
persistent identity: BBa C0053
display id: BBa_C0053
version: 1
was generated by: iGEM to SBOL conversion
description: c2 repressor from Salmonella phage P22 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage P22
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The P22 c2 repressor protein coding sequence is a 720 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the P22 c2 regulatory sequence, BBa_R0053. The sequence contains a LVA tag for faster degredation.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000) References (unparsed) here: Vander Byl,C. and Kropinski,A.M. Sequence of the genome of Salmonella bacteriophage P22 J. Bacteriol. 182 (22), 6472-6481 (2000)
star: true
creator: Maia Mahoney
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cII p22
sequence annotation: BBa_C0053
sequence annotation: LVA
sequence annotation: stop
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0053_sequence/1

cII p22
type: SequenceAnnotation
persistent identity: annotation1747
display id: annotation1747
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0053/annotation1747/range1747/1
type: Range
persistent identity: range1747
display id: range1747
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 648
orientation: inline

BBa_C0053
type: SequenceAnnotation
persistent identity: annotation7036
display id: annotation7036
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0053/annotation7036/range7036/1
type: Range
persistent identity: range7036
display id: range7036
version: 1
top level: c2 P22
direction: direction/forward
start: 1
end: 687
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1750
display id: annotation1750
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0053/annotation1750/range1750/1
type: Range
persistent identity: range1750
display id: range1750
version: 1
top level: c2 P22
direction: direction/forward
start: 649
end: 681
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1751
display id: annotation1751
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0053/annotation1751/range1751/1
type: Range
persistent identity: range1751
display id: range1751
version: 1
top level: c2 P22
direction: direction/forward
start: 682
end: 687
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213993
display id: annotation2213993
version: 1
top level: c2 P22
location: https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0053/annotation2213993/range2213993/1
type: Range
persistent identity: range2213993
display id: range2213993
version: 1
top level: c2 P22
direction: direction/forward
start: 688
end: 712
orientation: inline

BBa_I746365
type: ComponentDefinition
persistent identity: BBa I746365
display id: BBa_I746365
version: 1
was generated by: iGEM to SBOL conversion
description: PLL promoter from P4 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: P4 phage genome sequence
owned by: james
owned by: myers
mutable description: This is the PLL promoter taken from the P4 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PLL promoter.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PLL
sequence: https://synbiohub.org/public/igem/BBa_I746365_sequence/1

PLL
type: SequenceAnnotation
persistent identity: annotation1943788
display id: annotation1943788
version: 1
top level: BBa_I746365
location: https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I746365/annotation1943788/range1943788/1
type: Range
persistent identity: range1943788
display id: range1943788
version: 1
top level: BBa_I746365
direction: direction/forward
start: 1
end: 92
orientation: inline

BBa_I746351
type: ComponentDefinition
persistent identity: BBa I746351
display id: BBa_I746351
version: 1
was generated by: iGEM to SBOL conversion
description: pag activator from PSP3 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The pag activator taken from PSP3 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: PSP3 pag
sequence: https://synbiohub.org/public/igem/BBa_I746351_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943868
display id: annotation1943868
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746351/annotation1943868/range1943868/1
type: Range
persistent identity: range1943868
display id: range1943868
version: 1
top level: BBa_I746351
direction: direction/forward
start: 1
end: 12
orientation: inline

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943870
display id: annotation1943870
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746351/annotation1943870/range1943870/1
type: Range
persistent identity: range1943870
display id: range1943870
version: 1
top level: BBa_I746351
direction: direction/forward
start: 19
end: 237
orientation: inline

PSP3 pag
type: SequenceAnnotation
persistent identity: annotation1943869
display id: annotation1943869
version: 1
top level: BBa_I746351
location: https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746351/annotation1943869/range1943869/1
type: Range
persistent identity: range1943869
display id: range1943869
version: 1
top level: BBa_I746351
direction: direction/forward
start: 19
end: 19
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1106007_sequence/1
type: Sequence
persistent identity: BBa K1106007 sequence
display id: BBa_K1106007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatcccccctgttgcgtctgctgaaaatattcacaaaataaagcgtactagagattaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1106001_sequence/1
type: Sequence
persistent identity: BBa K1106001 sequence
display id: BBa_K1106001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatcccccctgttgcgtctgctgaaaatattcacaaaataaagcgtactagagattaaagaggagaaatactagatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1106002_sequence/1
type: Sequence
persistent identity: BBa K1106002 sequence
display id: BBa_K1106002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0053_sequence/1
type: Sequence
persistent identity: BBa C0053 sequence
display id: BBa_C0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746365_sequence/1
type: Sequence
persistent identity: BBa I746365 sequence
display id: BBa_I746365_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatcccccctgttgcgtctgctgaaaatattcacaaaataaagcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746351_sequence/1
type: Sequence
persistent identity: BBa I746351 sequence
display id: BBa_I746351_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcacatgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtacatttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcagaaaccaacaacagcaactttggctttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z