LOCUS       BBa_K1106008            1327 bp    DNA     linear       09-May-2024
DEFINITION  Pulse generator part 1
ACCESSION   BBa_K1106008
VERSION     BBa_K1106008.1  
FEATURES             Location/Qualifiers
     misc_feature    36..54
                     /label=ArsR binding site
     promoter        57..62
                     /label=-35 sequence
     promoter        80..85
                     /label=-10 sequence
     RBS             108..112
                     /label=rbs
     CDS             119..472
                     /label=arsR
     misc_feature    1..518
                     /label=BBa_J33201
     RBS             527..541
                     /label=RBS-1\Strong
     RBS             534..538
                     /label=RBS
     misc_feature    527..541
                     /label=BBa_B0030
     RBS             527..541
                     /label=BBa_B0030
     misc_feature    1127..1158
                     /label=LVA
     CDS             548..1126
                     /label=luxI
     misc_feature    548..1165
                     /label=BBa_C0061
     misc_feature    1166..1190
                     /label=Help:Barcodes
     CDS             548..1165
                     /label=BBa_C0061
     stem_loop       1210..1253
                     /label=stem_loop
     misc_feature    1199..1278
                     /label=BBa_B0010
     terminator      1199..1278
                     /label=BBa_B0010
     stem_loop       1294..1313
                     /label=T7 TE
     misc_feature    1320..1320
                     /label=stop
     polyA_site      1314..1327
                     /label=polya
     misc_feature    1287..1327
                     /label=BBa_B0012
     terminator      1287..1327
                     /label=BBa_B0012
     terminator      1199..1327
                     /label=BBa_B0015
ORIGIN
        1 ccaactcaaa attcacacct attaccttcc tctgcactta cacattcgtt aagtcatata
       61 tgtttttgac ttatccgctt cgaagagaga cactacctgc aacaatcagg agcgcaatat
      121 gtcatttctg ttacccatcc aattgttcaa aattcttgct gatgaaaccc gtctgggcat
      181 cgttttactg ctcagcgaac tgggagagtt atgcgtctgc gatctctgca ctgctctcga
      241 ccagtcgcag cccaagatct cccgccacct ggcattgctg cgtgaaagcg ggctattgct
      301 ggaccgcaag caaggtaagt gggttcatta ccgcttatca ccgcatattc cagcatgggc
      361 ggcgaaaatt attgatgagg cctggcgatg tgaacaggaa aaggttcagg cgattgtccg
      421 caacctggct cgacaaaact gttccgggga cagtaagaac atttgcagtt aaaaatttag
      481 ctaaacacat atgaattttc agatgtgttt tatccgggta ctagagatta aagaggagaa
      541 atactagatg actataatga taaaaaaatc ggattttttg gcaattccat cggaggagta
      601 taaaggtatt ctaagtcttc gttatcaagt gtttaagcaa agacttgagt gggacttagt
      661 tgtagaaaat aaccttgaat cagatgagta tgataactca aatgcagaat atatttatgc
      721 ttgtgatgat actgaaaatg taagtggatg ctggcgttta ttacctacaa caggtgatta
      781 tatgctgaaa agtgtttttc ctgaattgct tggtcaacag agtgctccca aagatcctaa
      841 tatagtcgaa ttaagtcgtt ttgctgtagg taaaaatagc tcaaagataa ataactctgc
      901 tagtgaaatt acaatgaaac tatttgaagc tatatataaa cacgctgtta gtcaaggtat
      961 tacagaatat gtaacagtaa catcaacagc aatagagcga tttttaaagc gtattaaagt
     1021 tccttgtcat cgtattggag acaaagaaat tcatgtatta ggtgatacta aatcggttgt
     1081 attgtctatg cctattaatg aacagtttaa aaaagcagtc ttaaatgctg caaacgacga
     1141 aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc
     1201 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1261 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1321 gtttata
//