BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1109001
1
BBa_K1109001
LqsR promoter
2013-09-22T11:00:00Z
2015-05-08T01:09:10Z
Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.) LqsR promoter region was amplified by PCR as a biobrick.
LqsR is the gene encoding response regulator component of the legionella quorum sensing (lqs). LqsR promoter region is controlled by its own response regulator LqsR.
false
false
_1420_
0
11226
9
Not in stock
false
This part is a new biobrick with prefix and suffix regeions.
false
Dr. ??zlem Darcansoy İşeri
BBa_K1109003
1
BBa_K1109003
LqsR promoter with RBS
2013-09-22T11:00:00Z
2015-05-08T01:09:10Z
Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid (Cellular Microbiology 9(12):2903-2920, 2007.) LqsR promoter region was amplified by PCR as a biobrick, and ligated with RBS (BBa_B0034).
LqsR is the gene encoding response regulator component of the legionella quorum sensing (lqs). LqsR promoter region is controlled by its own response regulator LqsR. This composite part contains RBS downstream of the promoter region. When any ORF is cloned to the downstream of this composite part, it can be expressed in response to legionella auto-inducer (LAI-I).
false
false
_1420_
0
11226
9
It's complicated
false
LqsR promoter part is a new biobrick with prefix and suffix regions, and RBS (BBa_B0034) was ligated to downstream of it.
false
Dr. ??zlem Darcansoy İşeri
component2358476
1
BBa_B0034
component2358474
1
BBa_K1109001
annotation2358476
1
BBa_B0034
range2358476
1
275
286
annotation2358474
1
BBa_K1109001
range2358474
1
1
266
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K1109001_sequence
1
gagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggata
BBa_K1109003_sequence
1
gagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggatatactagagaaagaggagaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z