BBa_K1109002 1 BBa_K1109002 lqsS-hdeD-lqsR coding region 2013-09-22T11:00:00Z 2015-05-08T01:09:10Z Legionella pneumophila genomic lqs gene cluster was cloned into pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.) LqsR and LqsS is the gene encoding response regulator and sensor kinase components of the legionella quorum sensing (lqs). false false _1420_ 0 11224 9 Not in stock false LqsR,hdeD and lqsS regions were amplified by PCR. Coding regions has unsuitable cutting areas so we amplified this region with one restriction site; speI. false Berkhan GEN?? BBa_K823008 1 BBa_K823008 Anderson promoter J23106 2012-09-07T11:00:00Z 2015-07-07T11:47:05Z Partsregistry Released HQ 2013 Anderson promoter J23106 without RFP in backbone vector pSB1C3 to easily fuse the promoter with other reporters e.g. the lux operon. false false _1081_ 4206 12081 9 In stock false no considerations false Korinna Kraft annotation2182529 1 -10 box range2182529 1 24 29 annotation2182530 1 J23106 range2182530 1 1 35 annotation2182528 1 -35 box range2182528 1 1 6 BBa_K1109006 1 BBa_K1109006 lqs response unite 2013-09-22T11:00:00Z 2015-05-08T01:09:10Z Source of the subparts are the 2013 DNA Distribution Kit and pNT-1 plasmid(Cellular Microbiology 9(12):2903-2920, 2007.) LqsR and LqsS are the genes encoding response regulator and sensor kinase components of the legionella quorum sensing (lqs) system, respectively. This composite part contains a constitutive promote region BBa_K823008 in the upstream these genes. When this composite ???lqs response unit??? is cloned into E. coli, the bacteria can express two components of the Legionella QS i.e; cell surface sensor kinase and cytosolic DNA binding component response regulator constitutively. false false _1420_ 0 11224 9 It's complicated false The LqsR and LqsS genes have illegal restriction enzyme cutting sites inside their coding regions. So, BBa_K1109002 composite part was amplified with primers having SpeI cutting sites, and cloned into the downstream of a constiutive promoter in the pSB1C3. false Berkhan GEN?? component2358762 1 BBa_K823008 component2358763 1 BBa_K1109002 annotation2358762 1 BBa_K823008 range2358762 1 1 35 annotation2358763 1 BBa_K1109002 range2358763 1 44 3292 BBa_K1109002_sequence 1 attaattcagaaacatttcctctgcatctaactcttccaggtattggtgataagaaagaattttatccaggcgcacatcgaacaaaacagccccttgcacataagcataaaaataggtttcatcagaaacaaatcggttggcaggaaccaaacaattcgaccattcattccattgaggtgtcactaaattagattgccaaaaacaaggaattttttctccattatgcaattggtccaatacatctccactagctccattatccaaagccaaatcatagtgtaactgcatatccgcttcgtttttcacgattaaaaaactgatgttggcatcatcatccagcatcaaaaagctacctgagttatcggccagataaaattcaacgattcccttttccctgcataaacgccagaataactcagcatattttttgtcatgtaaacaatttggcgatgtcaccgaaagcatgcgtacaatcatatcagacatgctttgaaaatattgtaactgcaggtcatggatgctttgagtaatgagactggcgacatccggatcactctttttgatgtatctatgaattaatccctcattaaaagcagcgattgccagcttctcatctgcttgcccagttaataatatttttttaatattagtatcttcgattcgcctgcaaaactctaatccatccatacccggcattgcataatcaacaacgactactgaaatttcagaaaatcgtcgagagttataaacctcggcatgaatagcagccaaatcaagattaatggtatgattagttagtgggcaattttttgcttctgtgtattcactgagacaacgttcacttaataattccagttcacagcgtttttgtttaatacaatctaaagcttcaaaaggggaatcaaatacacggtacgccaaaccctcatccagctgcaatacgaaattgagcaagaaatcccgactgtcgtcgacaaaaaccgcagtgcttggaaagtaacaggtaggtattgagaaatgttgcatggctcctcctgagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggatattgatatggctaattcacaagaatttaaccaacccccagtggatgagttaaaacgaaactggggatggattttaggatttggaatatttttccttatccttggctgtgttggtcttggaatggtagttggtctaaccttggtaagtatgttctttttcggtgccttattaattgttgggggaataagtcacattatagacgtatttaaatacaaagagtggaaaggaatgatatggcaggcgcttattgcagtgttgtatattgcagcgggatgcattgttttgtatgacccgtttttagcatctactctaatcacagcttttctggcagcggtgcttattgtaattggtttaacaagaatgattatggcttttgccttaaaagattccacaggatggggatggttgttattggccggaatcaccgccattattcttgggatattaattctaatgcaatggccagttagcgggctgtggatcattgggctttttatcgcgattgaattgatcgtcactggttggacatatatttttatcgctatctcagtaaaaacggccaaactctagcagaagagaatcacaggagctctctaactcaataccatccttacgtcttttaagacgaatgatggtatgttcaacagaaaaccaattttaaaccgagcctggaaaactcagtataaacaaagcgtattcgccctctttagattcacaaacaatatcaccaccaaacgcattcataactaatttacaaaatgataaaccaatgcctgttcccataaaggtagtagtatagaaatgattaaataatcgatttaattgttgtttgctcatcccttttgctgtgtctttaaaatacaaataatgatacttatcgccagattcagtccaaatattaatctccccacgctgtgccattgcgataacataaagtgcattttttattaaattaaataaaatatgctgcattaacaacttagagcctttaaattgaaaatcaccagtccatgtgattaatgttctttctcttggtgactgaaatgggtatctggtaatagcctcagacaaacattctgctatagaacaagtctctaatatgcaattttgcaaaatattatcgcgacttgccttgactaataacatatcaatgatcgtattagcataatcaatttcactaacaattcgattgctgactcccccaagctgtaataacaatctgtcacgtaaaggtgaggatattaactcttgttttttagccaattggtaaccatcaatcaatcttggcaaatattggactaaagcttgagcgccgctctttattcctaacaatggtgaacgcaattcatgggcaatcatgcctgcagcagcagccatgccagccaatttttgttgttgcaacatggctgttttataatttaaagtagaacctgcaataatggtaaatatgatcaccaatgtcatttgtatatgctcttctccaaaatacatttgcggagaaacaagataataacttacaagggctaaactaaaccccagtatcaacacaatggataaactcaataaatcaacgagcaatacaagtaaaaatacaccacacaacaaagacatcgcagaaataacgcttgcttgattcattaaaaataaaaaagtaaaaaaatatggcaaagtaaacaaaagagttaaaaaccaataccaagataaatattgcttccattttaatggccaataaggagtaagcatcaatcccaaacctaaaaggcttccaataagacggagtggcaggttttcataaggctgaggcaaccagaaagcccaaataacataaaataaaggaaatcccacaaaagctatcgcgcctactgcaaccaattgatgagcagaattagacaaactacgctgcattgattcatctaaatgtttcactattttttttagttgtgacat BBa_K823008_sequence 1 tttacggctagctcagtcctaggtatagtgctagc BBa_K1109006_sequence 1 tttacggctagctcagtcctaggtatagtgctagctactagagattaattcagaaacatttcctctgcatctaactcttccaggtattggtgataagaaagaattttatccaggcgcacatcgaacaaaacagccccttgcacataagcataaaaataggtttcatcagaaacaaatcggttggcaggaaccaaacaattcgaccattcattccattgaggtgtcactaaattagattgccaaaaacaaggaattttttctccattatgcaattggtccaatacatctccactagctccattatccaaagccaaatcatagtgtaactgcatatccgcttcgtttttcacgattaaaaaactgatgttggcatcatcatccagcatcaaaaagctacctgagttatcggccagataaaattcaacgattcccttttccctgcataaacgccagaataactcagcatattttttgtcatgtaaacaatttggcgatgtcaccgaaagcatgcgtacaatcatatcagacatgctttgaaaatattgtaactgcaggtcatggatgctttgagtaatgagactggcgacatccggatcactctttttgatgtatctatgaattaatccctcattaaaagcagcgattgccagcttctcatctgcttgcccagttaataatatttttttaatattagtatcttcgattcgcctgcaaaactctaatccatccatacccggcattgcataatcaacaacgactactgaaatttcagaaaatcgtcgagagttataaacctcggcatgaatagcagccaaatcaagattaatggtatgattagttagtgggcaattttttgcttctgtgtattcactgagacaacgttcacttaataattccagttcacagcgtttttgtttaatacaatctaaagcttcaaaaggggaatcaaatacacggtacgccaaaccctcatccagctgcaatacgaaattgagcaagaaatcccgactgtcgtcgacaaaaaccgcagtgcttggaaagtaacaggtaggtattgagaaatgttgcatggctcctcctgagcaaaacgttccaaagttatatcctttctatgaacttcaaataacctgtggctccatcatcagaacctggctaacttgtgaataaagtaacatgattgtttattataaccaaattatttaggttttatattcaccgggctttttggagtaagtattcaaaataagcgctcttaaaatcaaagctcaaaattagacaagttattgaatataagtctatattattaaataagtaccttatcttagctaagaaatcaaaaaaggatattgatatggctaattcacaagaatttaaccaacccccagtggatgagttaaaacgaaactggggatggattttaggatttggaatatttttccttatccttggctgtgttggtcttggaatggtagttggtctaaccttggtaagtatgttctttttcggtgccttattaattgttgggggaataagtcacattatagacgtatttaaatacaaagagtggaaaggaatgatatggcaggcgcttattgcagtgttgtatattgcagcgggatgcattgttttgtatgacccgtttttagcatctactctaatcacagcttttctggcagcggtgcttattgtaattggtttaacaagaatgattatggcttttgccttaaaagattccacaggatggggatggttgttattggccggaatcaccgccattattcttgggatattaattctaatgcaatggccagttagcgggctgtggatcattgggctttttatcgcgattgaattgatcgtcactggttggacatatatttttatcgctatctcagtaaaaacggccaaactctagcagaagagaatcacaggagctctctaactcaataccatccttacgtcttttaagacgaatgatggtatgttcaacagaaaaccaattttaaaccgagcctggaaaactcagtataaacaaagcgtattcgccctctttagattcacaaacaatatcaccaccaaacgcattcataactaatttacaaaatgataaaccaatgcctgttcccataaaggtagtagtatagaaatgattaaataatcgatttaattgttgtttgctcatcccttttgctgtgtctttaaaatacaaataatgatacttatcgccagattcagtccaaatattaatctccccacgctgtgccattgcgataacataaagtgcattttttattaaattaaataaaatatgctgcattaacaacttagagcctttaaattgaaaatcaccagtccatgtgattaatgttctttctcttggtgactgaaatgggtatctggtaatagcctcagacaaacattctgctatagaacaagtctctaatatgcaattttgcaaaatattatcgcgacttgccttgactaataacatatcaatgatcgtattagcataatcaatttcactaacaattcgattgctgactcccccaagctgtaataacaatctgtcacgtaaaggtgaggatattaactcttgttttttagccaattggtaaccatcaatcaatcttggcaaatattggactaaagcttgagcgccgctctttattcctaacaatggtgaacgcaattcatgggcaatcatgcctgcagcagcagccatgccagccaatttttgttgttgcaacatggctgttttataatttaaagtagaacctgcaataatggtaaatatgatcaccaatgtcatttgtatatgctcttctccaaaatacatttgcggagaaacaagataataacttacaagggctaaactaaaccccagtatcaacacaatggataaactcaataaatcaacgagcaatacaagtaaaaatacaccacacaacaaagacatcgcagaaataacgcttgcttgattcattaaaaataaaaaagtaaaaaaatatggcaaagtaaacaaaagagttaaaaaccaataccaagataaatattgcttccattttaatggccaataaggagtaagcatcaatcccaaacctaaaaggcttccaataagacggagtggcaggttttcataaggctgaggcaaccagaaagcccaaataacataaaataaaggaaatcccacaaaagctatcgcgcctactgcaaccaattgatgagcagaattagacaaactacgctgcattgattcatctaaatgtttcactattttttttagttgtgacat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z