LOCUS       BBa_K1109007            2372 bp    DNA     linear       25-Nov-2024
DEFINITION  FepA L8T Mutant -composite of Large Diffusion pore for E. coli 
            outer membrane coding sequence transc
ACCESSION   BBa_K1109007
VERSION     BBa_K1109007.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=-35 box
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=J23106
     promoter        1..35
                     /label=BBa_K823008
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..2269
                     /label=BBa_I746200
     misc_feature    44..2269
                     /label=BBa_K1109000
     stem_loop       2285..2304
                     /label=T7 TE
     misc_feature    2311..2311
                     /label=stop
     polyA_site      2305..2318
                     /label=polya
     misc_feature    2278..2318
                     /label=BBa_B0012
     terminator      2278..2318
                     /label=BBa_B0012
     stem_loop       2339..2361
                     /label=stem_loop
     misc_feature    2327..2372
                     /label=BBa_B0011
     terminator      2327..2372
                     /label=BBa_B0011
     terminator      2278..2372
                     /label=BBa_B0014
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaacaag aagattcatt ccctggcctt gttggtcaat ctggggattt atggggtagc
      121 gcaggcacaa gagccgaccg atactcctgt ttcacatgac gatactattg tcgttaccgc
      181 cgccgagcag aacttacagg cgcctggcgt ttcgaccatc accgcagatg aaatccgcaa
      241 aaacccggtt gcccgcgatg tgtcgaagat catccgtacc atgccaggcg ttaacctgac
      301 cggtaactcc accagtggtc agcgtgggaa taaccgacag attgatattc gcggtatggg
      361 tccggaaaac acgctgattt tgattgacgg caagccggta agcagccgta actcggtgcg
      421 tcagggctgg cgtggcgagc gcgatacccg tggtgatact tcctgggtgc cacctgaaat
      481 gattgaacgt attgaagttc tgcgtggtcc ggcagctgcg cgttatggca acggcgcggc
      541 gggcggcgtg gttaacatca ttaccaaaaa aggcagcggc gagtggcacg gctcctggga
      601 cgcatatttc aatgcgccag aacataaaga ggaaggtgcc accaaacgca ctaactttag
      661 cctgaccggt ccgctgggcg acgagttcag cttccgtttg tatggcaacc tcgacaaaac
      721 ccaggctgac gcgtgggata tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc
      781 cacgacgtta ccagccgggc gcgaaggggt aatcaacaaa gatattaatg gcgtggtgcg
      841 ctgggatttc gcgccattgc aatcgctgga actggaagca ggttacagcc gccagggtaa
      901 cctgtatgcg ggcgacaccc agaataccaa ctccgattcc tatacccgct cgaaatatgg
      961 cgatgaaacc aaccgtctgt atcgccagaa ctacgcgctg acctggaacg gtggctggga
     1021 taacggcgtg accaccagca actgggtgca gtacgaacac acccgtaact cgcgtattcc
     1081 ggaaggtctg gcgggcggta ccgaagggaa atttaacgaa aaagcgacac aggatttcgt
     1141 cgatatcgat cttgatgacg tgatgctgca cagcgaagtt aacctgccga ttgatttcct
     1201 cgttaaccag acgctgacgc tgggtacgga gtggaatcag caacggatga aggacttaag
     1261 ttccaacacc caggcactga ccggaacgaa taccggtggc gctattgatg gcgtgagtac
     1321 caccgaccgt agcccgtatt caaaagcaga aattttctcg ctgtttgccg aaaacaacat
     1381 ggagctgact gacagcacca tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt
     1441 cggcaataac tggagcccgg cgctgaacat atcgcaaggt ttaggcgatg acttcacgct
     1501 gaaaatgggc atcgcccgtg cttataaagc gccgagcctg taccagacta acccgaacta
     1561 cattctctac agtaaaggtc agggttgcta tgccagcgcg ggcggctgct atctgcaagg
     1621 taacgatgac ctgaaagcag aaaccagcat caacaaagag attggtctgg agttcaaacg
     1681 cgacgggtgg ctggcgggcg tcacctggtt ccgtaacgat tatcgcaata agattgaagc
     1741 gggctatgtg gctgtagggc aaaattgtga aacagcggtg gttgaaggtc tggaaggatc
     1801 gttaaacgta ccggttagcg aaacggtgat gtggaccaat aacatcactt atatgctgaa
     1861 gagtgaaaac aaaaccacgg gcgaccgttt gtcgatcatc ccggagtata cgttgaactc
     1921 aacgctgagc tggcaggcac gggaagattt gtcgatgcaa acgaccttca cctggtacgg
     1981 caagcagcag ccgaagaagt acaactataa aggtcagcca gcggttggac cggaaaccaa
     2041 agaaattagt ccttacagca ttgttggcct gagcgcgacc tgggatgtga cgaagaatgt
     2101 cagtctgacc ggcggcgtgg acaatctgtt cgacaaacgt ttgtggcgtg cgggtaatgc
     2161 ccagaccacg ggcgatttgg caggggccaa ctatatcgcc ggtgccgggg cgtataccta
     2221 taacgagccg ggacgtacgt ggtatatgag cgtaaacacc cacttctgat actagagtca
     2281 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa
     2341 gccagattat taatccggct tttttattat tt
//