BBa_K1111007 1 BBa_K1111007 MMP2 under the control of the pBAD/araC arabinose inducible promoter 2013-09-18T11:00:00Z 2015-05-08T01:09:10Z The MMP2 sequence is from Homo Sapiens This construct can induce expression of MMP2, a gelatinase upon addition of Arabinose to the milieu. This enzyme can then degrade gelatin false false _1422_ 0 16588 9 It's complicated false The MMP2 gene is expressed when arabinose is added. false Luisa Spisak, Marcelle Isaline Arrigo component2355423 1 BBa_K1111006 component2355422 1 BBa_I0500 annotation2355422 1 BBa_I0500 range2355422 1 1 1210 annotation2355423 1 BBa_K1111006 range2355423 1 1217 2798 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498843 1 AraC range1498843 1 1 1079 annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 BBa_K1111006 1 BBa_K1111006 Matrix metalloprotease 2 (MMP2) 2013-09-18T11:00:00Z 2015-05-08T01:09:10Z Homo Sapiens This enzyme is supposed to degrade gelatin false false _1422_ 0 16588 9 Not in stock false In order to activate the enzyme a special buffer is needed. We also added a his tag to the protein to purify it. false Luisa Spisak, Marcelle Isaline Arrigo BBa_K1111007_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagatgcaccaccaccaccaccacaagggaaataaaattttatacattttaggtacaggcatctttgttggaagttcatgtctattttcttcactttttgtagccgcagaagaacaagtttattcagaaagtgaagtttcaacagttttatcgaagttggaaaaggaggcaatttctgaggcagctgctgaacaatatacggttgtagatcgaaaagaagacgcgtgggggatgaagcatcttaagttagaaaagcaaacggaaggcgttactgttgattcagataatgtgattattcatttagataaaaacggtgcagtaacaagtgttacaggaaatccagttgatcaagttgtgaaaattcaatcggttgatgcaatcggtgaagaaggagttaaaaaaattgttgcttctgataatccagaaactaaagatcttgtctttttagctattgacaaacgtgtaaataatgaagggcaattattttataaagtcagagtaacttcttcaccaactggtgaccccgtatcattggtttataaagtgaacgctacagatggaacaattatggaaaaacaagatttaacggaacatgtcggtagtgaagtaacgttaaaaaactcttttcaagtaacgtttaatgtaccagttgaaaaaagcaatacgggaattgctttacacggaacggataacacaggggtttaccatgcagtagttgatggcaaaaataattattctattattcaagcgccatcactagcgacattaaatcagaatgctattgacgcctatacgcatggaaaatttgtgaaaacatattatgaagatcatttccaacgacacagtattgatgatcgagggatgcccatcttgtcagttgttgatgaacaacatccagatgcttatgacaatgctttttgggatggaaaagcaatgcgttatggtgaaacaagtacaccaacaggaaaaacgtatgcttcctctttagatgtagttggtcatgaaatgacacatggtgtgacggaacatactgccggtttagaatatttaggacaatcaggtgccttgaatgaatcttattctgatttgatgggttatattatttcgggtgcatctaatccagaaattggtgcggatactcagagtgttgaccgaaaaacaggtattcgaaatttacaaacgccaagtaaacacggacaaccagaaaccatggctcaatacgacgatcgagcacggtataaaggaacgccttattatgatcaaggcggtgttcattataacagtggaattattaatcggattggttacaccattatccagaacttaggcattgaaaaagcacagactattttctacagctcgttagtaaattacttaacacctaaagcacaattcagtgatgctcgtgatgcgatgcttgctgctgcaaaagttcaatatggcgatgaagcagcttcagtggtgtcagcagcctttaactctgctggaatcggagctaaagaagacattcaggtaaaccaaccaagtgaatctgttctggtcaatgaaggtgaaaatttgtattttcaatctggtggttgaatga BBa_K1111006_sequence 1 atgcaccaccaccaccaccacaagggaaataaaattttatacattttaggtacaggcatctttgttggaagttcatgtctattttcttcactttttgtagccgcagaagaacaagtttattcagaaagtgaagtttcaacagttttatcgaagttggaaaaggaggcaatttctgaggcagctgctgaacaatatacggttgtagatcgaaaagaagacgcgtgggggatgaagcatcttaagttagaaaagcaaacggaaggcgttactgttgattcagataatgtgattattcatttagataaaaacggtgcagtaacaagtgttacaggaaatccagttgatcaagttgtgaaaattcaatcggttgatgcaatcggtgaagaaggagttaaaaaaattgttgcttctgataatccagaaactaaagatcttgtctttttagctattgacaaacgtgtaaataatgaagggcaattattttataaagtcagagtaacttcttcaccaactggtgaccccgtatcattggtttataaagtgaacgctacagatggaacaattatggaaaaacaagatttaacggaacatgtcggtagtgaagtaacgttaaaaaactcttttcaagtaacgtttaatgtaccagttgaaaaaagcaatacgggaattgctttacacggaacggataacacaggggtttaccatgcagtagttgatggcaaaaataattattctattattcaagcgccatcactagcgacattaaatcagaatgctattgacgcctatacgcatggaaaatttgtgaaaacatattatgaagatcatttccaacgacacagtattgatgatcgagggatgcccatcttgtcagttgttgatgaacaacatccagatgcttatgacaatgctttttgggatggaaaagcaatgcgttatggtgaaacaagtacaccaacaggaaaaacgtatgcttcctctttagatgtagttggtcatgaaatgacacatggtgtgacggaacatactgccggtttagaatatttaggacaatcaggtgccttgaatgaatcttattctgatttgatgggttatattatttcgggtgcatctaatccagaaattggtgcggatactcagagtgttgaccgaaaaacaggtattcgaaatttacaaacgccaagtaaacacggacaaccagaaaccatggctcaatacgacgatcgagcacggtataaaggaacgccttattatgatcaaggcggtgttcattataacagtggaattattaatcggattggttacaccattatccagaacttaggcattgaaaaagcacagactattttctacagctcgttagtaaattacttaacacctaaagcacaattcagtgatgctcgtgatgcgatgcttgctgctgcaaaagttcaatatggcgatgaagcagcttcagtggtgtcagcagcctttaactctgctggaatcggagctaaagaagacattcaggtaaaccaaccaagtgaatctgttctggtcaatgaaggtgaaaatttgtattttcaatctggtggttgaatga BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z