LOCUS       BBa_K1111012            2097 bp    DNA     linear       23-Dec-2024
DEFINITION  Ice Nucleation Protein fused to Streptavidin Alive
ACCESSION   BBa_K1111012
VERSION     BBa_K1111012.1  
FEATURES             Location/Qualifiers
     promoter        2..20
                     /label=T7
     misc_feature    249..270
                     /label=CAP binding site
     promoter        285..315
                     /label=Lac
     misc_feature    323..339
                     /label=LacZ 
     misc_feature    623..1555
                     /label=BBa_K523013 INP
     misc_feature    1556..1606
                     /label=BBa_K523013 Linker 
     misc_feature    1607..1990
                     /label=BBa_K1111010
     misc_feature    1991..1993
                     /label=stop
     misc_feature    2064..2091
                     /label=T7 Terminator
     CDS             623..1993
                     /label=Fusion protein
     misc_feature    623..625
                     /label=INP start
     misc_feature    1607..1609
                     /label=Streptavidin start
ORIGIN
        1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc
       61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
      121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
      181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat
      361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat
      601 atttcgcgta aggaaatcca ttatgggatc tatgaccctg gataaagcgc tggtgctgcg
      661 cacttgtgcc aataatatgg ccgaccattg tggcctgatc tggcctgctt ctggtacagt
      721 tgaaagccgc tattggcagt ctacacgtcg tcacgaaaac gggctggttg ggctgctgtg
      781 gggtgccgga acatccgcct ttctgagcgt tcatgccgat gctcgttgga ttgtttgtga
      841 ggttgctgtt gccgacatca ttagcctgga agaaccgggt atggtgaaat ttcctcgtgc
      901 tgaagtggtc catgttggtg atcgtattag cgcctcccat ttcatttcag ctcgtcaggc
      961 tgatccagca agcacatcaa caagcacctc taccagtaca ctgacaccaa tgccaaccgc
     1021 tatccctact cctatgccgg ctgtagcctc tgttactctg cctgtggccg aacaagctcg
     1081 ccacgaagtc tttgacgttg cctctgtgtc tgctgctgct gctcctgtta acactctgcc
     1141 tgttaccacg cctcaaaatc tgcaaacggc gacctatggc tcaacactga gtggtgataa
     1201 ccattctcgt ctgattgccg ggtatggctc taacgaaacc gccggaaacc attctgatct
     1261 gatcggtggt cacgattgta cgctgatggc aggtgatcaa tctcgtctga ccgctggcaa
     1321 aaattccgtt ctgaccgctg gggcacgttc taaactgatc gggagcgaag gctctactct
     1381 gtccgccgga gaagattcta ccctgatctt tcgtctgtgg gatggtaaac gttatcgtca
     1441 gctggtcgct cgtacaggtg aaaatggtgt ggaagccgac attccgtatt atgtgaatga
     1501 ggacgatgat attgtggaca aaccggacga agatgatgat tggatcgaag tgaaaggtac
     1561 tagcagcagc attgcgagca gcagcccgag cagcgtggcg ggatctatgg ctgaagctgg
     1621 tatcaccggc acctggtaca accagctggg atccaccttc atcgttaccg ctggtgctga
     1681 cggtgctctg accggtacct acgaatccgc tgttggtaac gctgaatcta gatacgttct
     1741 gaccggtcgt tacgactccg ctccggctac cgacggttcc ggaaccgctc tgggttggac
     1801 cgttgcttgg aaaaacaact accgtaacgc tcactccgct accacctggt ctggccagta
     1861 cgttggtggt gctgaagctc gtatcaacac ccagtggttg ttgacctccg gcaccaccga
     1921 agctaacgcg tggaaatcca ccctggttgg tcacgacacc ttcaccaaag ttaaaccgtc
     1981 cgctgcttcc taacatatca taacggagtg atcgcaatgg gatctgttgt ttgtcggtga
     2041 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//