LOCUS       BBa_K1111016            2844 bp    DNA     linear       26-Nov-2024
DEFINITION  INP_Streptavidin_EYFP Fusion Protein
ACCESSION   BBa_K1111016
VERSION     BBa_K1111016.1  
FEATURES             Location/Qualifiers
     promoter        2..20
                     /label=T7 promoter
     misc_feature    249..270
                     /label=CAP binding site 
     promoter        285..315
                     /label=Lac
     misc_feature    323..339
                     /label=LacZ
     misc_feature    623..1555
                     /label=BBa_K523013 INP
     misc_feature    623..625
                     /label=INP start
     misc_feature    1556..1573
                     /label=PCR added INP-Strep Linker
     misc_feature    1574..1576
                     /label=Streptavidin start
     misc_feature    1574..1957
                     /label=BBa_K283010 without stop codon
     misc_feature    1958..2008
                     /label=Strep-EFYP Linker
     misc_feature    2009..2778
                     /label=BBa_K523013 INP
     misc_feature    2009..2011
                     /label=INP start
     misc_feature    2776..2778
                     /label=INP stop
     misc_feature    2811..2838
                     /label=T7 terminator
     CDS             623..2778
                     /label=Fusion Protein
ORIGIN
        1 ttaatacgac tcactatagg gagaggaggt acgatctgct ggggcaaacc agcgtggacc
       61 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
      121 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
      181 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      241 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
      301 ccggctcgta tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat
      361 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      421 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      481 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
      541 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgat actagctgat
      601 atttcgcgta aggaaatcca ttatgggatc tatgaccctg gataaagcgc tggtgctgcg
      661 cacttgtgcc aataatatgg ccgaccattg tggcctgatc tggcctgctt ctggtacagt
      721 tgaaagccgc tattggcagt ctacacgtcg tcacgaaaac gggctggttg ggctgctgtg
      781 gggtgccgga acatccgcct ttctgagcgt tcatgccgat gctcgttgga ttgtttgtga
      841 ggttgctgtt gccgacatca ttagcctgga agaaccgggt atggtgaaat ttcctcgtgc
      901 tgaagtggtc catgttggtg atcgtattag cgcctcccat ttcatttcag ctcgtcaggc
      961 tgatccagca agcacatcaa caagcacctc taccagtaca ctgacaccaa tgccaaccgc
     1021 tatccctact cctatgccgg ctgtagcctc tgttactctg cctgtggccg aacaagctcg
     1081 ccacgaagtc tttgacgttg cctctgtgtc tgctgctgct gctcctgtta acactctgcc
     1141 tgttaccacg cctcaaaatc tgcaaacggc gacctatggc tcaacactga gtggtgataa
     1201 ccattctcgt ctgattgccg ggtatggctc taacgaaacc gccggaaacc attctgatct
     1261 gatcggtggt cacgattgta cgctgatggc aggtgatcaa tctcgtctga ccgctggcaa
     1321 aaattccgtt ctgaccgctg gggcacgttc taaactgatc gggagcgaag gctctactct
     1381 gtccgccgga gaagattcta ccctgatctt tcgtctgtgg gatggtaaac gttatcgtca
     1441 gctggtcgct cgtacaggtg aaaatggtgt ggaagccgac attccgtatt atgtgaatga
     1501 ggacgatgat attgtggaca aaccggacga agatgatgat tggatcgaag tgaaaggtag
     1561 cggcagcggt agcatggctg aagctggtat caccggcacc tggtacaacc agctgggatc
     1621 caccttcatc gttaccgctg gtgctgacgg tgctctgacc ggtacctacg aatccgctgt
     1681 tggtaacgct gaaagccgct acgttctgac cggtcgttac gactccgctc cggctaccga
     1741 cggttccgga accgctctgg gttggaccgt tgcttggaaa aacaactacc gtaacgctca
     1801 ctccgctacc acctggtctg gccagtacgt tggtggtgct gaagctcgta tcaacaccca
     1861 gtggttgttg acctccggca ccaccgaagc caacgcgtgg aaatccaccc tggttggtca
     1921 cgacaccttc accaaagtta aaccgtccgc tgcttccggt actagcagca gcattgcgag
     1981 cagcagcccg agcagcgtgg cgggatctat ggtgagcaag ggcgaggagc tgttcaccgg
     2041 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     2101 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     2161 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg
     2221 cttcgcccgc taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga
     2281 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     2341 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     2401 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     2461 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     2521 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     2581 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga
     2641 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     2701 tctcggcatg gacgagctgt acaagtaata atactagcaa catatcataa cggagtgatc
     2761 gcaatgggat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2821 cgggtgggcc tttctgcgtt tata
//