BBa_K1112001 1 BBa_K1112001 pGlnA + hmsHFRS operon 2013-09-09T11:00:00Z 2015-05-08T01:09:11Z The operon used comes from Xenorhabdus nematophila, a nematode-dependent parasite. This operon allows the formation of a biofilm on nematodes pathogenic to insects and it is thus key in this nematode-bacteria symbiotic association that kills insects. To control the expression of the operon, we used a promoter from the standard registry of parts, which was characterized by our team last year (see Part:BBa_K763003). The molecular mechanism works as follows: with low nitrogen in the media, the promoter is activated and a biofilm of E.coli over C.elegans should be triggered and with high nitrogen concentration, the biobrick is unactive and Pseudomonas will not form the biofilm. false false _1423_ 0 12039 9 It's complicated false We developed a nitrogen-gradation tube experiment, which means that we added different (NH4)2SO4 concentrations to each tube, so we can measure a gradation of the protein fluorescence. We used a synthetic medium for this. false Alba Iglesias Vilches annotation2366925 1 HmsH range2366925 1 211 2560 annotation2366928 1 HmsS range2366928 1 5969 6341 annotation2366923 1 pGlnA range2366923 1 1 210 annotation2366924 1 hmsHFRS operon (biofilm formation genes) range2366924 1 211 6341 annotation2366926 1 HmsF range2366926 1 2561 4597 annotation2366927 1 HmsR range2366927 1 4598 5968 BBa_K1112001_sequence 1 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igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z