LOCUS BBa_K1113401 5774 bp DNA linear 22-Dec-2024 DEFINITION Targeting sequence for the delivery of the LacZ gene to the Carboxysome ACCESSION BBa_K1113401 VERSION BBa_K1113401.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1237..2655 /label=BBa_K1113701 misc_feature 2664..2693 /label=BBa_K105012 misc_feature 5723..5723 /label=C->T CDS 2700..5774 /label=lacZ misc_feature 2700..2702 /label=START misc_feature 5772..5774 /label=STOP CDS 2700..5774 /label=BBa_I732005 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cagttaaaaa gtatagtgct 1261 ggtgtaaaag aataccggca gacctattgg atgccggaat acacaccgtt ggattccgac 1321 atccttgcat gcttcaaaat caccccacaa ccgggtgttg atcgcgaaga agccgcagcc 1381 gcggttgcag cagaatcttc aaccggcaca tggaccaccg tgtggaccga tttgctgacc 1441 gacatggact actacaaagg ccgtgcctat cgcattgaag acgtacccgg tgacgatgcg 1501 gcattctatg cctttatcgc ctacccaatc gacctgttcg aagaagggtc agttgttaac 1561 gtgtttacct cactggttgg taacgtgttc ggcttcaaag cggtacgcgg cctgcgtctg 1621 gaagatgttc gcttcccact cgcctacgtt aaaacctgtg gcggcccacc gcacggtatt 1681 caagtcgaac gcgacaagat gaacaaatat ggtcgcccac tgttgggttg caccatcaag 1741 ccaaaacttg gtttgtctgc gaaaaactac ggccgtgccg tatacgagtg cctccgtggc 1801 ggcctcgact tcactaaaga tgatgaaaac atcaattctc agccgttcat gcgctggcgc 1861 gatcgcttct tgttcgtaca agacgcgacc gaaactgctg aagcccaaac cggcgaacgc 1921 aaaggccatt acctcaacgt aacggcgcca actcctgaag aaatgtacaa gcgcgcagag 1981 tttgccaaag aaattggcgc gccaatcatt atgcacgact acatcaccgg tggcttcacg 2041 gccaacactg gcttggccaa gtggtgtcaa gacaacggcg tactgctgca catccaccgt 2101 gcgatgcatg cggttatcga ccgtaacccg aaccacggta ttcacttccg tgttctgacc 2161 aagattctgc gtttgtcggg tggcgatcac ctgcacaccg gtaccgttgt cggcaaactg 2221 gaaggcgacc gtgcctctac tctgggctgg attgatttgc tccgcgaatc gtttatccct 2281 gaagatcgct cgcgcggtat cttcttcgat caagactggg gttcaatgcc aggcgtattc 2341 gctgtggcct ctggtggtat tcacgtatgg cacatgcctg cgctggttaa catctttggt 2401 gacgactctg tcctccaatt cggtggcggt acgctgggtc atccatgggg caacgctgcc 2461 ggtgctgctg ccaaccgtgt tgctctggaa gcctgcgtag aagcgcgtaa ccaaggccgc 2521 gatatcgaaa aagaaggcaa agaaattctg actgctgctg cacagcacag cccagaactg 2581 aagattgcca tggaaacttg gaaagagatc aaattcgaat ttgacactgt cgacaaactc 2641 gacactcaaa atcgttacta gagggtgaaa atttgtattt tcaatctggt ggttactaga 2701 tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 2761 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 2821 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct 2881 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg 2941 aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct 3001 acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga 3061 cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc 3121 gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg 3181 gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg 3241 gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc 3301 aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta 3361 cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac 3421 tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt 3481 tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg 3541 atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac 3601 tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg 3661 gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg 3721 gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc 3781 atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga 3841 agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca 3901 cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca 3961 tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg 4021 taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga 4081 atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc 4141 cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt 4201 gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca 4261 tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc 4321 acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc 4381 cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg 4441 aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc 4501 agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag 4561 caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca 4621 gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg 4681 gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga 4741 ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg 4801 tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt 4861 ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc 4921 tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc 4981 gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc 5041 tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc 5101 gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag 5161 cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc 5221 acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg 5281 gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg 5341 cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat 5401 tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc 5461 tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg 5521 ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca 5581 gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag 5641 aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga 5701 gcccgtcagt atcggcggaa tttcagctga gcgccggtcg ctaccattac cagttggtct 5761 ggtgtcaaaa ataa //