LOCUS       BBa_K1113501            3460 bp    DNA     linear       26-Dec-2024
DEFINITION  GFP with a targeting sequence for delivery into the Carboxysome 
            and a ssrA degradation tag
ACCESSION   BBa_K1113501
VERSION     BBa_K1113501.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     CDS             1237..2655
                     /label=BBa_K1113701
     misc_feature    2664..2693
                     /label=BBa_K105012
     misc_feature    2700..2702
                     /label=start
     CDS             2700..3419
                     /label=superfolder GFP coding region
     misc_feature    3414..3419
                     /label=stop
     CDS             2700..3419
                     /label=BBa_I746916
     misc_feature    3428..3460
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    3428..3460
                     /label=BBa_M0052
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatgg cagttaaaaa gtatagtgct
     1261 ggtgtaaaag aataccggca gacctattgg atgccggaat acacaccgtt ggattccgac
     1321 atccttgcat gcttcaaaat caccccacaa ccgggtgttg atcgcgaaga agccgcagcc
     1381 gcggttgcag cagaatcttc aaccggcaca tggaccaccg tgtggaccga tttgctgacc
     1441 gacatggact actacaaagg ccgtgcctat cgcattgaag acgtacccgg tgacgatgcg
     1501 gcattctatg cctttatcgc ctacccaatc gacctgttcg aagaagggtc agttgttaac
     1561 gtgtttacct cactggttgg taacgtgttc ggcttcaaag cggtacgcgg cctgcgtctg
     1621 gaagatgttc gcttcccact cgcctacgtt aaaacctgtg gcggcccacc gcacggtatt
     1681 caagtcgaac gcgacaagat gaacaaatat ggtcgcccac tgttgggttg caccatcaag
     1741 ccaaaacttg gtttgtctgc gaaaaactac ggccgtgccg tatacgagtg cctccgtggc
     1801 ggcctcgact tcactaaaga tgatgaaaac atcaattctc agccgttcat gcgctggcgc
     1861 gatcgcttct tgttcgtaca agacgcgacc gaaactgctg aagcccaaac cggcgaacgc
     1921 aaaggccatt acctcaacgt aacggcgcca actcctgaag aaatgtacaa gcgcgcagag
     1981 tttgccaaag aaattggcgc gccaatcatt atgcacgact acatcaccgg tggcttcacg
     2041 gccaacactg gcttggccaa gtggtgtcaa gacaacggcg tactgctgca catccaccgt
     2101 gcgatgcatg cggttatcga ccgtaacccg aaccacggta ttcacttccg tgttctgacc
     2161 aagattctgc gtttgtcggg tggcgatcac ctgcacaccg gtaccgttgt cggcaaactg
     2221 gaaggcgacc gtgcctctac tctgggctgg attgatttgc tccgcgaatc gtttatccct
     2281 gaagatcgct cgcgcggtat cttcttcgat caagactggg gttcaatgcc aggcgtattc
     2341 gctgtggcct ctggtggtat tcacgtatgg cacatgcctg cgctggttaa catctttggt
     2401 gacgactctg tcctccaatt cggtggcggt acgctgggtc atccatgggg caacgctgcc
     2461 ggtgctgctg ccaaccgtgt tgctctggaa gcctgcgtag aagcgcgtaa ccaaggccgc
     2521 gatatcgaaa aagaaggcaa agaaattctg actgctgctg cacagcacag cccagaactg
     2581 aagattgcca tggaaacttg gaaagagatc aaattcgaat ttgacactgt cgacaaactc
     2641 gacactcaaa atcgttacta gagggtgaaa atttgtattt tcaatctggt ggttactaga
     2701 tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat tctggtggaa ctggatggtg
     2761 atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga aggtgacgca actaatggta
     2821 aactgacgct gaagttcatc tgtactactg gtaaactgcc ggtaccttgg ccgactctgg
     2881 taacgacgct gacttatggt gttcagtgct ttgctcgtta tccggaccat atgaagcagc
     2941 atgacttctt caagtccgcc atgccggaag gctatgtgca ggaacgcacg atttccttta
     3001 aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt tgaaggcgat accctggtaa
     3061 accgcattga gctgaaaggc attgacttta aagaagacgg caatatcctg ggccataagc
     3121 tggaatacaa ttttaacagc cacaatgttt acatcaccgc cgataaacaa aaaaatggca
     3181 ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg cagcgtgcag ctggctgatc
     3241 actaccagca aaacactcca atcggtgatg gtcctgttct gctgccagac aatcactatc
     3301 tgagcacgca aagcgttctg tctaaagatc cgaacgagaa acgcgatcat atggttctgc
     3361 tggagttcgt aaccgcagcg ggcatcacgc atggtatgga tgaactgtac aaatgatgat
     3421 actagaggct gctaacgacg aaaactacgc tgacgcttct
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