BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_I746916 1 BBa_I746916 superfolder GFP coding sequence 2008-09-29T11:00:00Z 2015-08-31T04:08:05Z Superfolder GFP was originally described by: Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006 This version was synthesised de novo (by Geneart). This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). It carries the following amino acid changes with respect to mut3 GFP (E0040), the currently most commonly used GFP in the registry: S30R, Y39N, F64L, G65T, F99S, N105T, Y145F, M153T, V163A, I171V, A206V Its in-vivo properties are considerably improved with respect to mut3 - it develops fluorescence about 3fold faster than mut3 GFP and reaches 4fold higher absolute fluorescence levels. Fluorescenct colonies can be identified with the naked eye even without UV or blue light illumination (that is to say the amount of blue light in normal daylight or lablight is sufficient). Additionally it is more stable in vitro and refolds faster after in vitro denaturation with respect to mut3 GFP. Note: Superfolder GFP is available in constructs driven by the pBAD and T7 promoters: part numbers I746908 and I746909 respectively. Additionally 6-his tagged versions for protein purification exist: I746914 (pBAD driven) and I746915 (T7 driven). false false _116_ 0 2122 9 It's complicated false Codon optimisation before de novo synthesis was carried out for both, E.coli and Bacillus subtilis. false Stefan Milde annotation1977535 1 stop range1977535 1 715 720 annotation1977533 1 start range1977533 1 1 3 annotation1977534 1 superfolder GFP coding region range1977534 1 1 720 BBa_K1113701 1 BBa_K1113701 Targeting sequence for the delivery to the Carboxysome 2013-09-17T11:00:00Z 2015-05-08T01:09:11Z The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley) This targeting sequence consist in the large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme and it???s constructed to deliver proteins of interest to the Carboxysome false false _1424_ 0 17949 9 Not in stock false - false Belen Cespedes BBa_M0052 1 BBa_M0052 AANDENYADAS (moderately fast) SsrA degradation tag. 2007-12-05T12:00:00Z 2015-05-08T01:13:51Z This variation of the SsrA tag was studied by McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. SsrA tags are prevalent in E. coli. This sequence codes for the amino acid sequence AANDENYADAS, a slower variation of the WT SsrA tag sequence, which, when fused to the C-terminal of proteins, will make the protein susceptible to moderately fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~1 Vmax/ [Clpx6] min-1 from (1). See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240. false false _11_ 0 2398 11 Not in stock false C-terminal tag. Degradation rate is moderately fast. Variations of this sequence yield different degradation rates(see Parts BBa_M0050, BBa_M0051, BBa_M0053). Sequence derived from reverse translation of AANDENYADAS sequence using codon usage optimized for E. coli. Three C-terminal aa's (DAS in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX. See sources on the main part page for further information about the mechanism of the system. NOTE: this sequence has only the amino acid sequence for the tag and bears NO STOP CODONS, so be sure to include them if you use this sequence. false Felix Moser annotation1958882 1 AANDENYADAS SsrA degradation tag range1958882 1 1 33 BBa_K1113501 1 BBa_K1113501 GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag 2013-09-17T11:00:00Z 2015-05-08T01:09:11Z The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley). All the rest are bricks available in the Registry This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (BBa_K1113701) which deliver into the carboxysome and the ssrA degradation tag (BBa_M0052) which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will ???protect??? the fluorescens, in any other case this will be degraded by the protease false false _1424_ 0 17949 9 It's complicated false This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vector false Belen Cespedes component2353298 1 BBa_K105012 component2353302 1 BBa_I746916 component2353294 1 BBa_I0500 component2353304 1 BBa_M0052 component2353296 1 BBa_B0034 component2353297 1 BBa_K1113701 annotation2353302 1 BBa_I746916 range2353302 1 2700 3419 annotation2353296 1 BBa_B0034 range2353296 1 1219 1230 annotation2353304 1 BBa_M0052 range2353304 1 3428 3460 annotation2353298 1 BBa_K105012 range2353298 1 2664 2693 annotation2353297 1 BBa_K1113701 range2353297 1 1237 2655 annotation2353294 1 BBa_I0500 range2353294 1 1 1210 BBa_K105012 1 BBa_K105012 10 aa flexible protein domain linker 2008-10-18T11:00:00Z 2015-05-08T01:08:52Z Oligos with a yeast optimized coding for the peptide GENLYFQSGG have been ordered <br\n> The amino acide sequence corresponds to the interdomaine linker of xxx. ''paper'' This is a 10 amino acides long linker peptide which can be used to join protein domains together in a flexible way. So fusion proteins with variable DNA-binding and activation or repression domains might be assembled. <br\n> false true _253_ 0 3313 9 In stock true To enable the fusion with other domains in frame the vector of this BioBrick has no base pair in between the restriction side and the BioBrick. Furthermore, this coding sequence does not include a start codon.<br\n> For more information about this issus, see:<br\n> Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." <br\n> DSpace http://hdl.handle.net/1721.1/32535 (2006). true Manuel Gersbacher, Katja Karstens BBa_K1113701_sequence 1 atggcagttaaaaagtatagtgctggtgtaaaagaataccggcagacctattggatgccggaatacacaccgttggattccgacatccttgcatgcttcaaaatcaccccacaaccgggtgttgatcgcgaagaagccgcagccgcggttgcagcagaatcttcaaccggcacatggaccaccgtgtggaccgatttgctgaccgacatggactactacaaaggccgtgcctatcgcattgaagacgtacccggtgacgatgcggcattctatgcctttatcgcctacccaatcgacctgttcgaagaagggtcagttgttaacgtgtttacctcactggttggtaacgtgttcggcttcaaagcggtacgcggcctgcgtctggaagatgttcgcttcccactcgcctacgttaaaacctgtggcggcccaccgcacggtattcaagtcgaacgcgacaagatgaacaaatatggtcgcccactgttgggttgcaccatcaagccaaaacttggtttgtctgcgaaaaactacggccgtgccgtatacgagtgcctccgtggcggcctcgacttcactaaagatgatgaaaacatcaattctcagccgttcatgcgctggcgcgatcgcttcttgttcgtacaagacgcgaccgaaactgctgaagcccaaaccggcgaacgcaaaggccattacctcaacgtaacggcgccaactcctgaagaaatgtacaagcgcgcagagtttgccaaagaaattggcgcgccaatcattatgcacgactacatcaccggtggcttcacggccaacactggcttggccaagtggtgtcaagacaacggcgtactgctgcacatccaccgtgcgatgcatgcggttatcgaccgtaacccgaaccacggtattcacttccgtgttctgaccaagattctgcgtttgtcgggtggcgatcacctgcacaccggtaccgttgtcggcaaactggaaggcgaccgtgcctctactctgggctggattgatttgctccgcgaatcgtttatccctgaagatcgctcgcgcggtatcttcttcgatcaagactggggttcaatgccaggcgtattcgctgtggcctctggtggtattcacgtatggcacatgcctgcgctggttaacatctttggtgacgactctgtcctccaattcggtggcggtacgctgggtcatccatggggcaacgctgccggtgctgctgccaaccgtgttgctctggaagcctgcgtagaagcgcgtaaccaaggccgcgatatcgaaaaagaaggcaaagaaattctgactgctgctgcacagcacagcccagaactgaagattgccatggaaacttggaaagagatcaaattcgaatttgacactgtcgacaaactcgacactcaaaatcgt BBa_M0052_sequence 1 gctgctaacgacgaaaactacgctgacgcttct BBa_B0034_sequence 1 aaagaggagaaa BBa_K1113501_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagaaagaggagaaatactagatggcagttaaaaagtatagtgctggtgtaaaagaataccggcagacctattggatgccggaatacacaccgttggattccgacatccttgcatgcttcaaaatcaccccacaaccgggtgttgatcgcgaagaagccgcagccgcggttgcagcagaatcttcaaccggcacatggaccaccgtgtggaccgatttgctgaccgacatggactactacaaaggccgtgcctatcgcattgaagacgtacccggtgacgatgcggcattctatgcctttatcgcctacccaatcgacctgttcgaagaagggtcagttgttaacgtgtttacctcactggttggtaacgtgttcggcttcaaagcggtacgcggcctgcgtctggaagatgttcgcttcccactcgcctacgttaaaacctgtggcggcccaccgcacggtattcaagtcgaacgcgacaagatgaacaaatatggtcgcccactgttgggttgcaccatcaagccaaaacttggtttgtctgcgaaaaactacggccgtgccgtatacgagtgcctccgtggcggcctcgacttcactaaagatgatgaaaacatcaattctcagccgttcatgcgctggcgcgatcgcttcttgttcgtacaagacgcgaccgaaactgctgaagcccaaaccggcgaacgcaaaggccattacctcaacgtaacggcgccaactcctgaagaaatgtacaagcgcgcagagtttgccaaagaaattggcgcgccaatcattatgcacgactacatcaccggtggcttcacggccaacactggcttggccaagtggtgtcaagacaacggcgtactgctgcacatccaccgtgcgatgcatgcggttatcgaccgtaacccgaaccacggtattcacttccgtgttctgaccaagattctgcgtttgtcgggtggcgatcacctgcacaccggtaccgttgtcggcaaactggaaggcgaccgtgcctctactctgggctggattgatttgctccgcgaatcgtttatccctgaagatcgctcgcgcggtatcttcttcgatcaagactggggttcaatgccaggcgtattcgctgtggcctctggtggtattcacgtatggcacatgcctgcgctggttaacatctttggtgacgactctgtcctccaattcggtggcggtacgctgggtcatccatggggcaacgctgccggtgctgctgccaaccgtgttgctctggaagcctgcgtagaagcgcgtaaccaaggccgcgatatcgaaaaagaaggcaaagaaattctgactgctgctgcacagcacagcccagaactgaagattgccatggaaacttggaaagagatcaaattcgaatttgacactgtcgacaaactcgacactcaaaatcgttactagagggtgaaaatttgtattttcaatctggtggttactagatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgatactagaggctgctaacgacgaaaactacgctgacgcttct BBa_I746916_sequence 1 atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K105012_sequence 1 ggtgaaaatttgtattttcaatctggtggt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z