BBa_K1113502 1 BBa_K1113502 GFP with a targeting sequence for delivery into the Carboxysome and a ssrA degradation tag 2013-09-17T11:00:00Z 2015-05-08T01:09:11Z The large subunits of the RuBisCo was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemistry, University of California, Berkeley). All the rest are bricks available in the Registry This part consist in the superfolder GFP (BBa_I746916) attached to two targeting sequences: The large subunit of the ribulose-1,5-bisphosphate carboxylase/oxygenase enzyme (BBa_K1113701) which deliver into the carboxysome and the ssrA degradation tag (BBa_M0052) which is a targeting sequence for degradation by the protease ClpXP. This construct is used as a reporter for the correct assembly of the Carboxysome, it works with the competition of the two targeting sequence, in the case of a correct assembly of the carboxysome the microcompartment will ???protect??? the fluorescens, in any other case this will be degraded by the protease false false _1424_ 0 17949 9 No part sequence false This part it constructed with a mutation in the Prefix (from ???cggccgc??? to ???atacctg???) which erase the NotI restriction site, this modification allow the correct design of primers for Gibson assembly. This part its also constructed on the pSB4K5 standard vector false Belen Cespedes igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z