LOCUS       BBa_K1114400             540 bp    DNA     linear       22-Nov-2024
DEFINITION  This is a MoClo level 0 destination vector.
ACCESSION   BBa_K1114400
VERSION     BBa_K1114400.1  
FEATURES             Location/Qualifiers
     misc_feature    14..19
                     /label=BsaI
     misc_feature    528..533
                     /label=BsaI
     misc_feature    1..6
                     /label=SpeI
     misc_feature    7..12
                     /label=SpeI
     misc_feature    535..540
                     /label=SpeI
     misc_feature    21..24
                     /label=MoClo Fusion Site A
     misc_feature    523..526
                     /label=MoClo Fusion Site B
     CDS             33..514
                     /label=alpha fragment of lacZ
     misc_feature    27..32
                     /label=BbsI
     misc_feature    515..520
                     /label=BbsI
ORIGIN
        1 actagtacta gtgggtctca ggagatgtct tctgcaccat atgcggtgtg aaataccgca
       61 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg
      121 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
      181 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
      241 gacggccagt gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg
      301 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      361 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      421 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      481 gccagctgca ttaatgaatc ggccaacgcg cggggaagac gttactagag acctactagt
//