BBa_K1114419
1
BBa_K1114419
Level 1 MoClo Destination Vector with E and F fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
iGEM Distribution Kit
===Design Notes===
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This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites E on the 5' side and site F on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="http://parts.igem.org/Part:pSB1K3">BBa_pSB1K3</a>.
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===Source===
iGEM Distribution Kit
===References===
false
false
_1425_
0
17243
9
It's complicated
false
===Design Notes===
<html>
This is a MoClo destination vector containing the lacZ alpha fragment for blue-white screening with fusion sites E on the 5' side and site F on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA; Y =TGTG.
Summary of modifications from original part:
Backbone is a modified version of pSB1K3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href="http://parts.igem.org/Part:pSB1K3">BBa_pSB1K3</a>.
</html>
===Source===
false
Jake Awtry
BBa_K1114419_sequence
1
actagtggaagacatgcttagagacctgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacccggggatcctctagagtcgacctgcaggcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcgggggtctctcgct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z