BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1114204
1
BBa_K1114204
C0080_CD, Moclo analogue of BBA_C0080
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
igem kit
araC operon in Moclo form
false
false
_1425_
0
13936
9
In stock
false
n'a
false
Shawn Jin
BBa_K1114006
1
BBa_K1114006
The MoClo format of BBa_J23104 with EB fusion sites.
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
iGEM Distribution Kit
<html>
This is the <a href=???http://2013.igem.org/Team:BostonU/MoCloChara???>MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part.
</html>
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
false
false
_1425_
0
17243
9
In stock
false
<html>
This is the <a href=???http://2013.igem.org/Team:BostonU/MoCloChara???>MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part.
</html>
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
false
Jake Awtry
BBa_K1114512
1
BBa_K1114512
Level 1 MoClo device that constitutively expresses araC
2013-09-11T11:00:00Z
2015-05-08T01:09:12Z
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits
<html>
pJ04B2C12_EF<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the repressor lacI. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114204">C0080_CD</a>, and <a href="http://parts.igem.org/Part:BBA_K783060">B0015_DF</a> within the <a href="http://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
false
_1425_
0
17243
9
It's complicated
false
<html>
pJ04B2C12_EF<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses the repressor lacI. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114204">C0080_CD</a>, and <a href="http://parts.igem.org/Part:BBA_K783060">B0015_DF</a> within the <a href="http://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
Jake Awtry
component2339310
1
BBa_K1114006
component2339312
1
BBa_K1114204
component2339319
1
BBa_K783020
component2339311
1
BBa_K1114107
annotation2339310
1
BBa_K1114006
range2339310
1
1
43
annotation2339312
1
BBa_K1114204
range2339312
1
152
1074
annotation2339319
1
BBa_K783020
range2339319
1
1083
1211
annotation2339311
1
BBa_K1114107
range2339311
1
52
143
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K1114107
1
BBa_K1114107
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
false
_1425_
0
8248
9
In stock
false
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
Traci Haddock
annotation2361728
1
MoClo Fusion Site C
range2361728
1
89
92
annotation2361727
1
MoClo Fusion Site B
range2361727
1
1
4
annotation2361724
1
BCD2
range2361724
1
5
88
BBa_K783020
1
BBa_K783020
MoClo converted version of BBa_B0015
2012-10-01T11:00:00Z
2015-05-08T01:13:20Z
BBa_B0015
Released HQ 2013
This is the MoClo converted version of BBa_B0015, which is a double terminator consisting of BBa_B0010 and BBa_B0012. It has two MoClo fusion sites flanking it.
false
false
_1038_
0
8248
9
In stock
false
We had to design the proper MoClo fusion sites to flank this part.
false
Traci Haddock
component2214216
1
BBa_B0010
component2214218
1
BBa_B0012
annotation2214216
1
BBa_B0010
range2214216
1
1
80
annotation2214218
1
BBa_B0012
range2214218
1
89
129
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1114204_sequence
1
aatgatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataaaggt
BBa_K1114006_sequence
1
gcttttgacagctagctcagtcctaggtattgtgctagctact
BBa_K1114512_sequence
1
gcttttgacagctagctcagtcctaggtattgtgctagctacttactagagtactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaatgatggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataaaggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K783020_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1114107_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z