LOCUS       BBa_K1114515             977 bp    DNA     linear       26-Dec-2024
DEFINITION  Level 1 MoClo device that constitutively expresse RFP
ACCESSION   BBa_K1114515
VERSION     BBa_K1114515.1  
FEATURES             Location/Qualifiers
     promoter        1..43
                     /label=BBa_K1114006
     RBS             56..139
                     /label=BCD2
     misc_feature    52..55
                     /label=MoClo Fusion Site B
     misc_feature    140..143
                     /label=MoClo Fusion Site C
     RBS             52..143
                     /label=BBa_K1114107
     CDS             156..836
                     /label=RFP
     misc_feature    152..155
                     /label=MoClo Fusion Site C
     misc_feature    837..840
                     /label=MoClo Fusion Site D
     misc_feature    152..840
                     /label=BBa_K1114211
     stem_loop       860..903
                     /label=stem_loop
     misc_feature    849..928
                     /label=BBa_B0010
     terminator      849..928
                     /label=BBa_B0010
     stem_loop       944..963
                     /label=T7 TE
     misc_feature    970..970
                     /label=stop
     polyA_site      964..977
                     /label=polya
     misc_feature    937..977
                     /label=BBa_B0012
     terminator      937..977
                     /label=BBa_B0012
     terminator      849..977
                     /label=BBa_K783020
ORIGIN
        1 gcttttgaca gctagctcag tcctaggtat tgtgctagct acttactaga gtactgggcc
       61 caagttcact taaaaaggag atcaacaatg aaagcaattt tcgtactgaa acatcttaat
      121 catgctaagg aggttttcta atgtactaga gaatgatggc ttcctccgag gatgttatca
      181 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      241 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      301 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      361 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      421 aaggtttcaa atgggaacgt gttatgaact tcgaagatgg tggtgttgtt accgttaccc
      481 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      541 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      601 gtatgtaccc ggaggatggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
      661 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
      721 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaggactaca
      781 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataaaggt
      841 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      901 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      961 gcctttctgc gtttata
//