BBa_K1114006
1
BBa_K1114006
The MoClo format of BBa_J23104 with EB fusion sites.
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
iGEM Distribution Kit
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This is the <a href=???http://2013.igem.org/Team:BostonU/MoCloChara???>MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part.
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Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
false
false
_1425_
0
17243
9
In stock
false
<html>
This is the <a href=???http://2013.igem.org/Team:BostonU/MoCloChara???>MoClo</a> formatted part of BBa_J23104 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_J23104">BBa_J23104</a> page for full information on the part.
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Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
false
Jake Awtry
BBa_K1114515
1
BBa_K1114515
Level 1 MoClo device that constitutively expresse RFP
2013-09-11T11:00:00Z
2015-05-08T01:09:12Z
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits
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pI134B2Rm_EF<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="http://parts.igem.org/Part:BBa_K783020">B0015_DF</a> within the <a href="http://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
false
_1425_
0
17243
9
It's complicated
false
<html>
pI134B2Rm_EF<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114006">J23104_EB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="http://parts.igem.org/Part:BBa_K783020">B0015_DF</a> within the <a href="http://parts.igem.org/Part:BBa_K1114419">DVL1_EF</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
Jake Awtry
component2339372
1
BBa_K1114006
component2339373
1
BBa_K1114107
component2339381
1
BBa_K783020
component2339374
1
BBa_K1114211
annotation2339372
1
BBa_K1114006
range2339372
1
1
43
annotation2339381
1
BBa_K783020
range2339381
1
849
977
annotation2339374
1
BBa_K1114211
range2339374
1
152
840
annotation2339373
1
BBa_K1114107
range2339373
1
52
143
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K1114211
1
BBa_K1114211
E1010m_CD
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
igem kit
Modified BBA_E0040 converted to Moclo with one cut site eliminated in the middle of the sequence.
false
false
_1425_
0
13936
9
In stock
false
n'a
false
Shawn Jin
annotation2361770
1
MoClo Fusion Site C
range2361770
1
1
4
annotation2361771
1
MoClo Fusion Site D
range2361771
1
686
689
annotation2361769
1
RFP
range2361769
1
5
685
BBa_K783020
1
BBa_K783020
MoClo converted version of BBa_B0015
2012-10-01T11:00:00Z
2015-05-08T01:13:20Z
BBa_B0015
Released HQ 2013
This is the MoClo converted version of BBa_B0015, which is a double terminator consisting of BBa_B0010 and BBa_B0012. It has two MoClo fusion sites flanking it.
false
false
_1038_
0
8248
9
In stock
false
We had to design the proper MoClo fusion sites to flank this part.
false
Traci Haddock
component2214218
1
BBa_B0012
component2214216
1
BBa_B0010
annotation2214216
1
BBa_B0010
range2214216
1
1
80
annotation2214218
1
BBa_B0012
range2214218
1
89
129
BBa_K1114107
1
BBa_K1114107
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
false
_1425_
0
8248
9
In stock
false
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
Traci Haddock
annotation2361724
1
BCD2
range2361724
1
5
88
annotation2361727
1
MoClo Fusion Site B
range2361727
1
1
4
annotation2361728
1
MoClo Fusion Site C
range2361728
1
89
92
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K1114006_sequence
1
gcttttgacagctagctcagtcctaggtattgtgctagctact
BBa_K783020_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1114107_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
BBa_K1114515_sequence
1
gcttttgacagctagctcagtcctaggtattgtgctagctacttactagagtactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaggatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaggactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1114211_sequence
1
aatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaggatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaggactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggt
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z