BBa_K1114005
1
BBa_K1114005
The MoClo format of BBa_J23104 with AB fusion sites.
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
iGEM Distribution Kit
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
</html>
false
false
_1425_
0
17243
9
In stock
false
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
</html>
false
Jake Awtry
annotation2361746
1
MoClo Fusion Site A
range2361746
1
1
4
annotation2361747
1
MoClo Fusion Site B
range2361747
1
40
43
annotation2361748
1
BBa_J23104
range2361748
1
5
39
BBa_K1114300
1
BBa_K1114300
This is the MoClo formatted part of BBa_B0015.
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
iGEM kit
This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114407 ???>BBa_</a>.
false
false
_1425_
0
17246
9
In stock
false
This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114407 ???>BBa_</a>.
false
Devina Desai
annotation2361811
1
MoClo Fusion Site D
range2361811
1
1
4
annotation2361813
1
BBa_B0015
range2361813
1
5
133
annotation2361812
1
MoClo Fusion Site E
range2361812
1
134
137
BBa_K1114516
1
BBa_K1114516
Level 1 MoClo Device that expresses tetR under pBAD
2013-09-11T11:00:00Z
2015-05-08T01:09:12Z
Construct of MoClo Level 0 parts derived from iGEM Distribution Kits
<html>
pI134B2C40_AE<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114202">C0040_CD</a>, and <a href="http://parts.igem.org/Part:BBa_K1114300">B0015_DE</a> within the <a href="http://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
false
_1425_
0
17243
9
It's complicated
false
<html>
pI134B2C40_AE<br><br>
This is a Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that expresses YFP under the repressible promoter lacI. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="http://parts.igem.org/Part:BBa_K1114107:Design">BCD2_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114202">C0040_CD</a>, and <a href="http://parts.igem.org/Part:BBA_K1114300">B0015_DE</a> within the <a href="http://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
Jake Awtry
component2339368
1
BBa_K1114202
component2339367
1
BBa_K1114107
component2339366
1
BBa_K1114005
component2339369
1
BBa_K1114300
annotation2339366
1
BBa_K1114005
range2339366
1
1
43
annotation2339369
1
BBa_K1114300
range2339369
1
825
961
annotation2339368
1
BBa_K1114202
range2339368
1
152
816
annotation2339367
1
BBa_K1114107
range2339367
1
52
143
BBa_K1114202
1
BBa_K1114202
C0040_CD, Moclo of BBA_C0040
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
igem kit
Moclo analogue of bba_c0040 with C and D fusion sites added
false
false
_1425_
0
13936
9
In stock
false
n/a
false
Shawn Jin
BBa_K1114107
1
BBa_K1114107
Bicistronic Design RBS 2, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites. This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
false
_1425_
0
8248
9
In stock
false
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 1 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
Traci Haddock
annotation2361728
1
MoClo Fusion Site C
range2361728
1
89
92
annotation2361727
1
MoClo Fusion Site B
range2361727
1
1
4
annotation2361724
1
BCD2
range2361724
1
5
88
BBa_K1114516_sequence
1
ggagttgacagctagctcagtcctaggtattgtgctagctacttactagagtactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataaaggttactagagaggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt
BBa_K1114300_sequence
1
aggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt
BBa_K1114005_sequence
1
ggagttgacagctagctcagtcctaggtattgtgctagctact
BBa_K1114202_sequence
1
aatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataaaggt
BBa_K1114107_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z