BBa_K1114701
1
BBa_K1114701
BioBrick of Level 1 MoClo RFP Reporter
2013-09-16T11:00:00Z
2015-05-08T01:09:12Z
Construct of MoClo Level 0 parts derived from iGEM Distribution Kit.
<html>
This is a BioBrick adaptation of the Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="http://parts.igem.org/Part:BBa_K1114108:Design">BCD8_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114211">E0040m_CD</a>, and <a href="http://parts.igem.org/Part:BBa_K783000">B0015_DE</a> within the <a href="http://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
false
_1425_
0
17243
9
It's complicated
false
<html>
This is a BioBrick adaptation of the Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="http://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="http://parts.igem.org/Part:BBa_K1114108:Design">BCD8_BC</a>, <a href="http://parts.igem.org/Part:BBa_K1114211">E0040m_CD</a>, and <a href="http://parts.igem.org/Part:BBA_K783000">B0015_DE</a> within the <a href="http://parts.igem.org/Part:BBa_K1114418">DVL1_AE</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.
</html>
false
Jake Awtry
component2361833
1
BBa_K1114005
component2361841
1
BBa_K1114211
component2361845
1
BBa_K1114300
component2361837
1
BBa_K1114108
annotation2361841
1
BBa_K1114211
range2361841
1
136
824
annotation2361837
1
BBa_K1114108
range2361837
1
44
135
annotation2361833
1
BBa_K1114005
range2361833
1
1
43
annotation2361845
1
BBa_K1114300
range2361845
1
825
961
BBa_K1114108
1
BBa_K1114108
Bicistronic design RBS 8, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
false
_1425_
0
8248
9
In stock
false
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
</html>
false
Traci Haddock
annotation2361725
1
MoClo Fusion Site B
range2361725
1
1
4
annotation2361723
1
BCD8
range2361723
1
5
88
annotation2361726
1
MoClo Fusion Site C
range2361726
1
89
92
BBa_K1114005
1
BBa_K1114005
The MoClo format of BBa_J23104 with AB fusion sites.
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
iGEM Distribution Kit
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
</html>
false
false
_1425_
0
17243
9
In stock
false
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of BBa_R0079 with EB fusion sites. See the <a href="http://parts.igem.org/Part:BBa_R0079">BBa_R0079</a> page for full information on the part.
Summary of modifications from original part:
Backbone has one added SpeI site in front of gene, BsaI sites, and 4bp fusion sites.
</html>
false
Jake Awtry
annotation2361748
1
BBa_J23104
range2361748
1
5
39
annotation2361746
1
MoClo Fusion Site A
range2361746
1
1
4
annotation2361747
1
MoClo Fusion Site B
range2361747
1
40
43
BBa_K1114211
1
BBa_K1114211
E1010m_CD
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
igem kit
Modified BBA_E0040 converted to Moclo with one cut site eliminated in the middle of the sequence.
false
false
_1425_
0
13936
9
In stock
false
n'a
false
Shawn Jin
annotation2361771
1
MoClo Fusion Site D
range2361771
1
686
689
annotation2361770
1
MoClo Fusion Site C
range2361770
1
1
4
annotation2361769
1
RFP
range2361769
1
5
685
BBa_K1114300
1
BBa_K1114300
This is the MoClo formatted part of BBa_B0015.
2013-09-06T11:00:00Z
2015-05-08T01:09:12Z
iGEM kit
This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114407 ???>BBa_</a>.
false
false
_1425_
0
17246
9
In stock
false
This is a Level 0 MoClo part with flanking sites D on the 5' side and site E on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Summary of modifications from original part:
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_ K1114407 ???>BBa_</a>.
false
Devina Desai
annotation2361813
1
BBa_B0015
range2361813
1
5
133
annotation2361811
1
MoClo Fusion Site D
range2361811
1
1
4
annotation2361812
1
MoClo Fusion Site E
range2361812
1
134
137
BBa_K1114300_sequence
1
aggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt
BBa_K1114005_sequence
1
ggagttgacagctagctcagtcctaggtattgtgctagctact
BBa_K1114108_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcatcggaccgtttctaatg
BBa_K1114211_sequence
1
aatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaggatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaggactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggt
BBa_K1114701_sequence
1
ggagttgacagctagctcagtcctaggtattgtgctagctacttactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcatcggaccgtttctaatgaatgatggcttcctccgaggatgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagatggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaggatggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaggactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaaggtaggtccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z