LOCUS       BBa_K1115009            1790 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive production (PCat) of dhlB and dhlA
ACCESSION   BBa_K1115009
VERSION     BBa_K1115009.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     RBS             55..61
                     /label=Shine-Dalgarno
     misc_feature    67..69
                     /label=Start
     CDS             67..825
                     /label=dhlB coding sequence
     misc_feature    826..828
                     /label=Opal Stop
     misc_feature    833..839
                     /label=HindIII
     RBS             842..849
                     /label=GsiB RBS (BBa_K143020)
     misc_feature    854..856
                     /label=Start
     CDS             854..1783
                     /label=dhlA coding sequence
     misc_feature    1784..1786
                     /label=Amber Stop
     misc_feature    47..1790
                     /label=BBa_K1115008
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagataa cagaaggagg
       61 aacgacatga taaaggcagt cgtgttcgac gcttacggca cgctcttcga cgtccagtcg
      121 gtggccgatg ccacagagca ggcgtatcct ggccggggcg attacatcac acaggtgtgg
      181 cggcagaagc agctggaata cagctggctc cgcgcgctga tggggcgcta tgccgacttc
      241 tggagcgtca cgcgggaggc gttggcctac accctcggaa cgctgggact ggagccggac
      301 aaggcctttc tcgccggcat ggcagaggcc tacaaccgcc tcacgcccta tcccgacgcc
      361 gcccgatgcc tcgcggagct ggcgccgctg aagcgcgcca tcctgtcaaa cggggcacca
      421 gacatgctgc gggcgctggt aggaaatgcg gccctgacgg acagcttcga cgcggtcatc
      481 agcgtcgatg tcaagcgcgt gttcaagccg caccccgagg cctatgcgct agtggaagag
      541 gtccttggcg taaagccggc ggaggtgctg ttcgtgtcct ccaacggctt cgacgtgggt
      601 ggcgcgaagt ctttcggctt ccgtgtcgcc cgcgtcgccc gcctgccgcg ggaggcgctc
      661 gcgcgcgaac tcgcctccgg gtccattgcg ccgctcacca tgttcaaggc gctcaggatg
      721 caggaggagg cctatgccga ggcgcctgat ttcgtggtgc ccgcccttgg cgacctgccg
      781 cggttggtcc gcgagatggc cggtgctcgt ctcgcgccag cggtgtgacg agaagcttgc
      841 taaaggaggc tctatgataa atgcaattcg caccccggac caacgcttca gcaatctcga
      901 tcagtatccg ttcagcccca actacctgga cgacctcccc ggctacccgg gattgcgggc
      961 acactacctc gacgagggca attctgacgc tgaagacgtt tttctctgcc ttcatggcga
     1021 gcccacctgg agttacctgt atcgcaagat gatcccggta tttgctgaat caggcgcacg
     1081 agttattgcg ccagactttt ttggattcgg aaaatccgac aagccagtag acgaagaaga
     1141 ctacaccttc gaatttcacc gcaacttcct gcttgcacta atcgaacggc ttgacttgcg
     1201 caacattacg ctggtcgttc aggactgggg cggatttttg gggctgacct taccgatggc
     1261 cgacccttcc cgcttcaagc gcctgatcat catgaacgcc tgcttgatga ccgacccggt
     1321 cacccagcct gcgtttagcg cctttgtcac ccagcctgcg gatggcttta ccgcctggaa
     1381 atacgatctg gttacgccat cagacctgcg ccttgaccag ttcatgaagc gttgggcgcc
     1441 cacactgacc gaagctgagg cctccgcgta tgctgcgcct ttccctgaca cttcctatca
     1501 ggctggtgta cgcaagtttc ccaagatggt cgcgcaacgc gaccaggcct gcatcgacat
     1561 ttcaaccgaa gcgatttcgt tctggcagaa cgactggaat ggccagacct tcatggccat
     1621 tggcatgaaa gacaaattgc tgggaccgga cgtcatgtat cctatgaagg cgctcattaa
     1681 tggctgcccg gaacccctcg aaatagcgga cgctggccat ttcgtacagg agtttggcga
     1741 gcaagtggct cgcgaggccc tgaaacactt tgccgagaca gaatagagga
//