LOCUS       BBa_K1115010            1806 bp    DNA     linear       23-Dec-2024
DEFINITION  Constitutive production (PTet)  of dhlB and dhlA
ACCESSION   BBa_K1115010
VERSION     BBa_K1115010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             71..77
                     /label=Shine-Dalgarno
     misc_feature    83..85
                     /label=Start
     CDS             83..841
                     /label=dhlB coding sequence
     misc_feature    842..844
                     /label=Opal Stop
     misc_feature    849..855
                     /label=HindIII
     RBS             858..865
                     /label=GsiB RBS (BBa_K143020)
     misc_feature    870..872
                     /label=Start
     CDS             870..1799
                     /label=dhlA coding sequence
     misc_feature    1800..1802
                     /label=Amber Stop
     misc_feature    63..1806
                     /label=BBa_K1115008
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agataacaga aggaggaacg acatgataaa ggcagtcgtg ttcgacgctt acggcacgct
      121 cttcgacgtc cagtcggtgg ccgatgccac agagcaggcg tatcctggcc ggggcgatta
      181 catcacacag gtgtggcggc agaagcagct ggaatacagc tggctccgcg cgctgatggg
      241 gcgctatgcc gacttctgga gcgtcacgcg ggaggcgttg gcctacaccc tcggaacgct
      301 gggactggag ccggacaagg cctttctcgc cggcatggca gaggcctaca accgcctcac
      361 gccctatccc gacgccgccc gatgcctcgc ggagctggcg ccgctgaagc gcgccatcct
      421 gtcaaacggg gcaccagaca tgctgcgggc gctggtagga aatgcggccc tgacggacag
      481 cttcgacgcg gtcatcagcg tcgatgtcaa gcgcgtgttc aagccgcacc ccgaggccta
      541 tgcgctagtg gaagaggtcc ttggcgtaaa gccggcggag gtgctgttcg tgtcctccaa
      601 cggcttcgac gtgggtggcg cgaagtcttt cggcttccgt gtcgcccgcg tcgcccgcct
      661 gccgcgggag gcgctcgcgc gcgaactcgc ctccgggtcc attgcgccgc tcaccatgtt
      721 caaggcgctc aggatgcagg aggaggccta tgccgaggcg cctgatttcg tggtgcccgc
      781 ccttggcgac ctgccgcggt tggtccgcga gatggccggt gctcgtctcg cgccagcggt
      841 gtgacgagaa gcttgctaaa ggaggctcta tgataaatgc aattcgcacc ccggaccaac
      901 gcttcagcaa tctcgatcag tatccgttca gccccaacta cctggacgac ctccccggct
      961 acccgggatt gcgggcacac tacctcgacg agggcaattc tgacgctgaa gacgtttttc
     1021 tctgccttca tggcgagccc acctggagtt acctgtatcg caagatgatc ccggtatttg
     1081 ctgaatcagg cgcacgagtt attgcgccag acttttttgg attcggaaaa tccgacaagc
     1141 cagtagacga agaagactac accttcgaat ttcaccgcaa cttcctgctt gcactaatcg
     1201 aacggcttga cttgcgcaac attacgctgg tcgttcagga ctggggcgga tttttggggc
     1261 tgaccttacc gatggccgac ccttcccgct tcaagcgcct gatcatcatg aacgcctgct
     1321 tgatgaccga cccggtcacc cagcctgcgt ttagcgcctt tgtcacccag cctgcggatg
     1381 gctttaccgc ctggaaatac gatctggtta cgccatcaga cctgcgcctt gaccagttca
     1441 tgaagcgttg ggcgcccaca ctgaccgaag ctgaggcctc cgcgtatgct gcgcctttcc
     1501 ctgacacttc ctatcaggct ggtgtacgca agtttcccaa gatggtcgcg caacgcgacc
     1561 aggcctgcat cgacatttca accgaagcga tttcgttctg gcagaacgac tggaatggcc
     1621 agaccttcat ggccattggc atgaaagaca aattgctggg accggacgtc atgtatccta
     1681 tgaaggcgct cattaatggc tgcccggaac ccctcgaaat agcggacgct ggccatttcg
     1741 tacaggagtt tggcgagcaa gtggctcgcg aggccctgaa acactttgcc gagacagaat
     1801 agagga
//