LOCUS       BBa_K1116002            1787 bp    DNA     linear       07-Dec-2025
DEFINITION  TRE--LacI with T21 and miR-FF3 target
ACCESSION   BBa_K1116002
VERSION     BBa_K1116002.1  
FEATURES             Location/Qualifiers
     promoter        2..251
                     /label=TRE
     CDS             425..1543
                     /label=LacI
     misc_feature    1555..1576
                     /label=T21
     misc_feature    1577..1598
                     /label=T21
     misc_feature    1599..1620
                     /label=T21
     misc_feature    1621..1642
                     /label=T21
     misc_feature    1650..1670
                     /label=FF3
     misc_feature    1676..1696
                     /label=FF3
     misc_feature    1702..1722
                     /label=FF3
     misc_feature    1728..1748
                     /label=FF3
ORIGIN
        1 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat
       61 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
      121 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
      181 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
      241 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
      301 tgaaccgtca gatcgcctgg agatttcgag ctcggtaccc ggggatcctc tagtcagctg
      361 acgcgtgcta gtggcgcgcc gaattcgacc caagtttgta caaaaaagca ggctgactcc
      421 caccatgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac
      481 cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga
      541 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
      601 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
      661 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
      721 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
      781 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
      841 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
      901 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
      961 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
     1021 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
     1081 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
     1141 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
     1201 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
     1261 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
     1321 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
     1381 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
     1441 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1501 gcagagcagc ctgaggcctc ctaagaagaa gaggaaggtt tgagcggccg caaatcaaca
     1561 tcagtctgat aagctatcaa catcagtctg ataagctatc aacatcagtc tgataagcta
     1621 tcaacatcag tctgataagc taaagcttaa acgatatggg ctgaatacaa taccaaacga
     1681 tatgggctga atacaatacc aaacgatatg ggctgaatac aataccaaac gatatgggct
     1741 gaatacaata ccgcaattgt ttcctttttt ttcctcaggg tacccag
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