BBa_K115001
1
BBa_K115001
RNA thermometer (ROSE)
2008-07-14T11:00:00Z
2015-05-08T01:09:27Z
This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
Released HQ 2013
This part is designed as a temperature inducible RBS. Only designed so far.
false
false
_223_
0
3007
9
In stock
true
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
true
O.M.J.A. Stassen
annotation1966928
1
Predicted Stem Loop
range1966928
1
39
71
annotation1966927
1
Predicted Stem Loop
range1966927
1
16
36
annotation1966898
1
Biobrick-Scar-adaptation
range1966898
1
74
79
annotation1966926
1
Predicted Stem Loop
range1966926
1
1
15
annotation1966896
1
SD
range1966896
1
90
95
annotation1966929
1
Predicted Stem Loop extending to startcodon
range1966929
1
72
96
BBa_K1117006
1
BBa_K1117006
J23119-K115001-GroEL
2013-09-14T11:00:00Z
2015-05-08T01:09:13Z
made from an ordered series of basic parts
trong constitutive promoter :j23119 42℃ RNA thermometer: k115001 HSP: GroEL
false
false
_1428_
0
16377
9
It's complicated
false
NO
false
Chun Li
component2346243
1
BBa_J23119
component2346250
1
BBa_K115001
component2346251
1
BBa_K1117002
annotation2346250
1
BBa_K115001
range2346250
1
44
139
annotation2346243
1
BBa_J23119
range2346243
1
1
35
annotation2346251
1
BBa_K1117002
range2346251
1
146
1768
BBa_K1117002
1
BBa_K1117002
GroEL
2013-09-14T11:00:00Z
2015-05-08T01:09:13Z
It is cloned from Thermoanaerobacter tengcongensis MB4.
GroEL(Hsp60) belongs to the chaperonin family of molecular chaperonescan. It could help proteins to fold properly with the presence of protein complex GroES(Hsp10).
false
false
_1428_
0
15735
9
It's complicated
false
No
false
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1117006_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagaggccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggattactagatggcaaaacagatcaaatacggtgaggaagcgcgcaaggctttagaaagaggagttaatgctgttgctaatactgtgaaggtaacactgggtccaagaggaaggaatgtagtacttgacaagaaatatggaactcctactgttaccaatgacggtgttacaattgcaagagaaattgaattagaagacccatttgagaatcaaggtgctcagcttttgaaagaggctgcaacgaagacaaatgatgtagcgggtgatggtacgactacagcaacccttctagcccaagtaatggttctggaagggcttaaaaaccttgctgcgggtgccaatccaatgctcttaagaagaggtatggcaaaggctgtggaagccgcagtcgagggtttaagaaggatttctaagcctattgataataaagagtcaattgcacatgtagctgctatttcagctgctgatgaggaaattggacagctcattgcagaagcaatggagaaagttggtaaagacggtgtcattacagttgaagagtcaaagactatcggtacaactcttgaagttgtagaaggtatgcagtttgacagaggttacatctcaccttacatggtaacagatgcagagaagatggaagcagtacttgaagagcctgtaatactcatcacagataaaaaactctctagtgtgcaagacttattgccattgctagagcaaatagtacagcacggaaagaaacttctcataattgctgatgatgtggaaggagaagctttggcaacattagttgttaacaagttaagaggtacattctcctgcgtggctgtaaaagctcctggttttggcgacagaagaaaggaaatgctccaggatattgctatcttgacaggtgggcaagtaatttctgaagagttgggttatgacttaaaagatgtgacattagatatgttaggccgtgcaagacaggtaaaagtcacaaaagaacatacaacgattgtaggtggagcaggtaatccagaagatatcaagaagagaattaaccagataaaggctcaaattgaagagactacttcagattacgatagagagaaattacaggagagacttgctaaactagctggcggcgtagcagttatccaagtaggtgctgcaacagagacagagctcaaagagaagaaacacaggattgaagatgcattagcagctacaaaagcagcagtagaagaaggtatagtgcctggtggtggagtagcacttcttaacgtaatagaagatgtgcagaaagtagttgactcattagaaggagacttcaagacaggggccaagatagtattgaaagctttagaagcacctgtaaggcagattgctgaaaacgcaggagttgatggttcaataatcgtagaaaagattaaagctgctaaagatccgaacttcggatacgatgcatacagagaagaatttacagatatgatcaagagaggtattatagacccgacaaaagttacaaggacagcacttcaaaatgctgcttcaattgcttcaatgatactcacaacagaggctatagtagtagatgtgccagagaaagaaaaaagcaatattcccggggcaggaatggacatgatgtaa
BBa_K115001_sequence
1
gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggat
BBa_K1117002_sequence
1
atggcaaaacagatcaaatacggtgaggaagcgcgcaaggctttagaaagaggagttaatgctgttgctaatactgtgaaggtaacactgggtccaagaggaaggaatgtagtacttgacaagaaatatggaactcctactgttaccaatgacggtgttacaattgcaagagaaattgaattagaagacccatttgagaatcaaggtgctcagcttttgaaagaggctgcaacgaagacaaatgatgtagcgggtgatggtacgactacagcaacccttctagcccaagtaatggttctggaagggcttaaaaaccttgctgcgggtgccaatccaatgctcttaagaagaggtatggcaaaggctgtggaagccgcagtcgagggtttaagaaggatttctaagcctattgataataaagagtcaattgcacatgtagctgctatttcagctgctgatgaggaaattggacagctcattgcagaagcaatggagaaagttggtaaagacggtgtcattacagttgaagagtcaaagactatcggtacaactcttgaagttgtagaaggtatgcagtttgacagaggttacatctcaccttacatggtaacagatgcagagaagatggaagcagtacttgaagagcctgtaatactcatcacagataaaaaactctctagtgtgcaagacttattgccattgctagagcaaatagtacagcacggaaagaaacttctcataattgctgatgatgtggaaggagaagctttggcaacattagttgttaacaagttaagaggtacattctcctgcgtggctgtaaaagctcctggttttggcgacagaagaaaggaaatgctccaggatattgctatcttgacaggtgggcaagtaatttctgaagagttgggttatgacttaaaagatgtgacattagatatgttaggccgtgcaagacaggtaaaagtcacaaaagaacatacaacgattgtaggtggagcaggtaatccagaagatatcaagaagagaattaaccagataaaggctcaaattgaagagactacttcagattacgatagagagaaattacaggagagacttgctaaactagctggcggcgtagcagttatccaagtaggtgctgcaacagagacagagctcaaagagaagaaacacaggattgaagatgcattagcagctacaaaagcagcagtagaagaaggtatagtgcctggtggtggagtagcacttcttaacgtaatagaagatgtgcagaaagtagttgactcattagaaggagacttcaagacaggggccaagatagtattgaaagctttagaagcacctgtaaggcagattgctgaaaacgcaggagttgatggttcaataatcgtagaaaagattaaagctgctaaagatccgaacttcggatacgatgcatacagagaagaatttacagatatgatcaagagaggtattatagacccgacaaaagttacaaggacagcacttcaaaatgctgcttcaattgcttcaatgatactcacaacagaggctatagtagtagatgtgccagagaaagaaaaaagcaatattcccggggcaggaatggacatgatgtaa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z