BBa_K115001
1
BBa_K115001
RNA thermometer (ROSE)
2008-07-14T11:00:00Z
2015-05-08T01:09:27Z
This sequence is taken from the Bradirhizobium Japonicum (BA000040.) as the 5'UTR (ROSE) of a heat shock protein (prfA).
Released HQ 2013
This part is designed as a temperature inducible RBS. Only designed so far.
false
false
_223_
0
3007
9
In stock
true
The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.
true
O.M.J.A. Stassen
annotation1966929
1
Predicted Stem Loop extending to startcodon
range1966929
1
72
96
annotation1966898
1
Biobrick-Scar-adaptation
range1966898
1
74
79
annotation1966926
1
Predicted Stem Loop
range1966926
1
1
15
annotation1966928
1
Predicted Stem Loop
range1966928
1
39
71
annotation1966927
1
Predicted Stem Loop
range1966927
1
16
36
annotation1966896
1
SD
range1966896
1
90
95
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1117004
1
BBa_K1117004
ThiF
2013-09-14T11:00:00Z
2015-05-08T01:09:13Z
It is cloned from Thermoanaerobacter tengcongensis MB4.
ThiF acts like an E1 ubiquitin-activating enzyme for ThiS, which is a ubiquitin-like protein in prokaryotes. The process of ubiquitination can target denatured proteins and stimulate their degradation.
false
false
_1428_
0
15735
9
It's complicated
false
No.
false
BBa_K1117007
1
BBa_K1117007
J23119-K115001-ThiF
2013-09-14T11:00:00Z
2015-05-08T01:09:13Z
made from an ordered series of basic parts
Strong constitutive promoter :j23119 42℃ RNA thermometer: k115001 HSP: THIF
false
false
_1428_
0
16377
9
It's complicated
false
NO
false
component2346240
1
BBa_K115001
component2346233
1
BBa_J23119
component2346241
1
BBa_K1117004
annotation2346233
1
BBa_J23119
range2346233
1
1
35
annotation2346240
1
BBa_K115001
range2346240
1
44
139
annotation2346241
1
BBa_K1117004
range2346241
1
146
751
BBa_K1117004_sequence
1
atgaatccttttgatgtaatgcttaaaaattattttgatgaaaagatgcttgacaagctttcaagagtaaaaattcttctcattggctgtggtggacttggttcaaatgttgcagtgatgcttataagaagtggtattaaaaacctcacaatagtcgattttgatgaggtagacatcttaaatctcaaccgacaaaattactttttacaccaggtaggagaaaaaaaggtattagcccttaaagatactctctttaaaatcaacccccacgcaaacatagcagccaaaaatataaaaattgatgagtcaaatttagatgagttaatccctcaacatgatatagttgtggaagctgttgacaatgaatttaccaaaacgctgatttttaaaaaagcccaaaaacataataaaaaagtggttttggcttcaggtgtcgcaggatttggtgattgcgaaaatattaaaataaaacggggtaaaaacttttcaattgtgggtgattttgttacatcgattaatgaaaagaaacctttggcacccaaagtcatggcagttgccgcaatacaagcagatgaagtattaaggatgataaatgagctggagtaa
BBa_K115001_sequence
1
gccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggat
BBa_K1117007_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagaggccgcgacaagcggtccgggcgccctaggggcccggcggagacgggcgccggaggtgtccgacgcctgctcgtcaagttcttgctccttggaggattactagatgaatccttttgatgtaatgcttaaaaattattttgatgaaaagatgcttgacaagctttcaagagtaaaaattcttctcattggctgtggtggacttggttcaaatgttgcagtgatgcttataagaagtggtattaaaaacctcacaatagtcgattttgatgaggtagacatcttaaatctcaaccgacaaaattactttttacaccaggtaggagaaaaaaaggtattagcccttaaagatactctctttaaaatcaacccccacgcaaacatagcagccaaaaatataaaaattgatgagtcaaatttagatgagttaatccctcaacatgatatagttgtggaagctgttgacaatgaatttaccaaaacgctgatttttaaaaaagcccaaaaacataataaaaaagtggttttggcttcaggtgtcgcaggatttggtgattgcgaaaatattaaaataaaacggggtaaaaacttttcaattgtgggtgattttgttacatcgattaatgaaaagaaacctttggcacccaaagtcatggcagttgccgcaatacaagcagatgaagtattaaggatgataaatgagctggagtaa
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z