BBa_K112010 1 BBa_K112010 a~T4 antiholin! in BBb 2008-09-02T11:00:00Z 2015-05-08T01:09:13Z <pre> PCR bx11/bx8 on Bjh1303 (329 bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI) Product is pBca1256-K112010 ---------------------------------------- bx11 Forward biobricking of {a~T4 Antiholin!} ccagtGAATTCatgAGATCTctttatggccttaaaagcaacagc bx8 Reverse biobricking of {a~T4 Antiholin!} cgttaGGATCCtcattcagtctccaatttaatgttc </pre> T4 antiholin gene ready to have an rbs attached to the front, with a stop codon, in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 It's complicated false N/A false Bing Xia BBa_K112010_sequence 1 ctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z