BBa_K112016
1
BBa_K112016
a~T4 lysozyme! in BBb
2008-09-02T11:00:00Z
2015-05-08T01:09:13Z
<pre>
PCR bx17/bx16 on Bjh1302 (530 bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI)
Product is pBca1256-K112016
----------------------------------------
bx17 Forward biobricking of {a~T4 Endolysin!}
ccagtGAATTCatgAGATCTtattatgaatatatttgaaatgttacgtatagatg
bx16 Reverse biobricking of {a~T4 Endolysin!}
cgttaGGATCCtagatttttatacgcgtcccaagtgcc
</pre>
T4 lysozyme gene ready to have an rbs attached in front, with a stop codon, in BBb format.
----
This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br>
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]
false
false
_224_
0
2998
9
It's complicated
false
N/A
false
Bing Xia
BBa_K112016_sequence
1
tattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z