BBa_K112903 1 BBa_K112903 b0015 terminator 2008-10-23T11:00:00Z 2015-05-08T01:09:19Z ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112311 1 BBa_K112311 {rbs.lambda holin!} in BBb format 2008-10-20T11:00:00Z 2015-05-08T01:09:16Z pSB3C6-Bjh1502 lambda holin with rbs site and stop codon in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 It's complicated true <pre> PCR al0012/al0008 on pSB3C6-Bjh1502 (369bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112311 ----------------------------------------------- al0012 Forward Biobricking of lambda holin with rbs site CCATAGAATTCatgAGATCTattgggggtaagacCtgaag al0008 Reverse Biobricking of lambda holin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc </pre> false Aron Lau BBa_K112906 1 BBa_K112906 PconG6 2008-10-23T11:00:00Z 2015-05-08T01:09:20Z false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112900 1 BBa_K112900 Pbad 2008-10-20T11:00:00Z 2015-05-08T01:09:19Z Arabinose induced promoter. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112999 1 scar BBb1 scar sequence 2008-10-20T11:00:00Z 2015-05-08T01:09:20Z This is the scar sequence of the BBb1 assembly scheme. For more information, refer to [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page]. false false _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112317 1 BBa_K112317 {rbs.lambda antiholin!} in BBb format 2008-10-20T11:00:00Z 2015-05-08T01:09:16Z pSB3C6-Bjh1503 lambda antiholin with native rbs site and stop codon. This part is actually in BBb format. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 It's complicated true <pre> PCR al0016/al0008 on pSB3C6-Bjh1503 (375bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112317 ----------------------------------------------- al0016 Forward Biobricking of lambda antiholin with rbs site CCATAGAATTCatgAGATCTccccttattgggggtaagacatg al0008 Reverse Biobricking of lambda antiholin with rbs site CGttaGGATCCttattgatttctaccatcttctactccggc </pre> false Aron Lau BBa_K112305 1 BBa_K112305 {rbs.lambda lysozyme!} in BBb format 2008-10-20T11:00:00Z 2015-05-08T01:09:16Z pSB3C6-Bjh1501 lambda lysozyme with native rbs site in BBb format. More information about the BBb Format is available at: [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false false _224_ 0 2999 9 It's complicated true <pre> PCR al0006/al0002 on pSB3C6-Bjh1501 (527bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+910, L) Product is pBca1256-K112305 ----------------------------------------------- al0006 Forward Biobricking of lambda lysozyme with rbs site CCATAGAATTCatgAGATCTaaaaaagccggagtagaagatgg al0002 Reverse Biobricking of lambda lysozyme with rbs site CGttaGGATCCtcatacatcaatctctctgaccgttccgcc </pre> false Aron Lau BBa_K112710 1 BBa_K112710 bca1041 2008-10-20T11:00:00Z 2015-05-08T01:09:18Z <pre> Construction of <Bca1041> basic part K112710 PCR of dv023/dv024 pSB1AG0-Bca1041 (122bp, EcoRI/BamHI/DpnI) Sub into pBca1256 (EcoRI/BamHI, 2472+122, L) Product is pBca1256-K112710 ----------------------------------------------- dv023 Forward biobricking of Bca1041 cgataGAATTCatgAGATCTgCcggcttatcGgtcagtttc dv024 Reverse biobricking of Bca1041 cgttaGGATCCatttcttttgggtatagcgtcgtgg </pre> The bca1041 terminator. ---- This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:<br> [http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BBb Standard Description Page] false true _224_ 0 2998 9 Not in stock false false Bing Xia BBa_K112020 1 BBa_K112020 Lambda phage lysis device 2008-10-23T11:00:00Z 2015-05-08T01:09:13Z Assembled using 2 antibiotic assembly from basic parts. Lysozyme and holin production are driven by pBad, and antiholin is driven by PconC6. false false _224_ 0 2998 9 It's complicated false false Bing Xia component1986755 1 BBa_K112906 component1986753 1 BBa_K112903 component1986748 1 BBa_K112999 component1986752 1 BBa_K112999 component1986749 1 BBa_K112305 component1986756 1 BBa_K112999 component1986757 1 BBa_K112317 component1986759 1 BBa_K112710 component1986751 1 BBa_K112311 component1986747 1 BBa_K112900 component1986758 1 BBa_K112999 component1986750 1 BBa_K112999 component1986754 1 BBa_K112999 annotation1986751 1 BBa_K112311 range1986751 1 1734 2071 annotation1986756 1 BBa_K112999 range1986756 1 2260 2265 annotation1986755 1 BBa_K112906 range1986755 1 2219 2259 annotation1986747 1 BBa_K112900 range1986747 1 1 1225 annotation1986748 1 BBa_K112999 range1986748 1 1226 1231 annotation1986749 1 BBa_K112305 range1986749 1 1232 1727 annotation1986750 1 BBa_K112999 range1986750 1 1728 1733 annotation1986758 1 BBa_K112999 range1986758 1 2610 2615 annotation1986757 1 BBa_K112317 range1986757 1 2266 2609 annotation1986753 1 BBa_K112903 range1986753 1 2078 2212 annotation1986752 1 BBa_K112999 range1986752 1 2072 2077 annotation1986759 1 BBa_K112710 range1986759 1 2616 2730 annotation1986754 1 BBa_K112999 range1986754 1 2213 2218 BBa_K112906_sequence 1 gatctgttactgctagctcagtcctaggcactttgctagcg BBa_K112305_sequence 1 aaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctattcactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatga BBa_K112317_sequence 1 ccccttattgggggtaagacatgaagctgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataa BBa_K112311_sequence 1 attgggggtaagacctgaagatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataa BBa_K112903_sequence 1 gatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatag BBa_K112020_sequence 1 aggattcttagctttcgctaggatgatttctggaattcatgagatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtctttactggctcttctcgctaccaacggtaaccccgcttattaaaagcattctgtacaagcgggacaaagcatgacaaaaacgcgtaacaagtgtcttatatcacggcgaaagttcaatggatatttgcacgctcactgcatgctagcattttatcgataagattaccgatcttacctgaagctggatctaaaaaagccggagtagaagatggtagaaatcaataatcaacgtaaggcgttcctcgatatgctggcgtggtcggagggaactgataacggacgtcagaaaaccagaaatcatggttatgacgtcattgtaggcggagagctattcactgattactccgatcaccctcgcaaacttgtcacgctaaacccaaaactcaaatcaacaggcgccggacgctaccagcttctttcccgttggtgggatgcctaccgcaagcagcttggcctgaaagacttctctccgaaaagtcaggacgctgtggcattgcagcagattaaggagcgtggcgctttacctatgattgatcgtggtgatatccgtcaggcaatcgaccgttgcagcaatatctgggcttcactgccgggcgctggttatggtcagttcgagcataaggctgacagcctgattgcaaaattcaaagaagcgggcggaacggtcagagagattgatgtatgaggatctattgggggtaagacctgaagatgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaggatctgatctccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagggatctgatctgttactgctagctcagtcctaggcactttgctagcgggatctccccttattgggggtaagacatgaagctgccagaaaaacatgacctgttggccgccattctcgcggcaaaggaacaaggcatcggggcaatccttgcgtttgcaatggcgtaccttcgcggcagatataatggcggtgcgtttacaaaaacagtaatcgacgcaacgatgtgcgccattatcgcctggttcattcgtgaccttctcgacttcgccggactaagtagcaatctcgcttatataacgagcgtgtttatcggctacatcggtactgactcgattggttcgcttatcaaacgcttcgctgctaaaaaagccggagtagaagatggtagaaatcaataaggatctgccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaat BBa_K112999_sequence 1 ggatct BBa_K112900_sequence 1 aggattcttagctttcgctaggatgatttctggaattcatgagatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagacgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagcaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcagggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtctttactggctcttctcgctaccaacggtaaccccgcttattaaaagcattctgtacaagcgggacaaagcatgacaaaaacgcgtaacaagtgtcttatatcacggcgaaagttcaatggatatttgcacgctcactgcatgctagcattttatcgataagattaccgatcttacctgaagct BBa_K112710_sequence 1 gccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z